Production of tannase by Aspergillus tamarii in submerged cultures
| Autor: | Wanessa X. Ribeiro, Antonio Roberto Giriboni Monteiro, Rosane Marina Peralta, Andréa Miura da Costa, Elaine Kato |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Multidisciplinary submerged cultures lcsh:Biotechnology Fungus Biology biology.organism_classification Tannase chemistry.chemical_compound enzyme tannase Enzyme chemistry Biochemistry Aspergillus tamarii tannins lcsh:TP248.13-248.65 Tannic acid Extracellular Inducer Gallic acid Food science fungal biomass Methyl gallate |
| Zdroj: | Brazilian Archives of Biology and Technology, Volume: 51, Issue: 2, Pages: 399-404, Published: APR 2008 Scopus-Elsevier Brazilian Archives of Biology and Technology, Vol 51, Iss 2, Pp 399-404 (2008) Brazilian Archives of Biology and Technology v.51 n.2 2008 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
| Popis: | The production of tannase by Aspergillus tamarii was studied in submerged cultures. The fungus produced an extracellular tannase after two days of growth in mineral medium containing tannic acid, gallic acid and methyl gallate as carbon source. The best result was obtained using gallic acid as inducer (20.6 U/ml). The production of enzyme was strongly repressed by the presence of glucose. Crude enzyme was optimally active at pH 5.0 and 30º C. The enzyme was stable in a large range of pH and up to the temperature of 45º C.A produção de tanase por um novo potencial produtor, o fungo filamentoso Aspergillus tamarii, foi parcialmente caracterizada neste estudo. O fungo produziu uma tanase extracelular em culturas submersas após 2 dias de crescimento em meio mineral contendo ácido tânico, ácido gálico ou metil galato como fonts de carbono. Os melhores resultados foram obtidos em culturas com ácido gálico (20,6 U/ml). A produção da enzima foi fortemente inibida por glicose. A enzima bruta foi otimamente ativa em pH 5,0 e a 30º C e estável em ampla faixa de pH e em temperaturas inferiores a 45ºC. |
| Databáze: | OpenAIRE |
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