In vitro reactivation of latent equid herpesvirus-1 from CD5+/CD8+ leukocytes indirectly by IL-2 or chorionic gonadotrophin
| Autor: | A S Hamblin, N. Edington, A Purewal, J Iqbal, D. J. Smith |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
chemical and pharmacologic phenomena
In situ hybridization CD8-Positive T-Lymphocytes CD5 Antigens Chorionic Gonadotropin Polymerase Chain Reaction Virus In vivo Virology Virus latency medicine Animals Humans Horses Cells Cultured Phytohaemagglutinin biology Pokeweed mitogen medicine.disease In vitro Virus Latency biology.protein Interleukin-2 Virus Activation Rabbits CD8 Herpesvirus 1 Equid |
| Zdroj: | Journal of General Virology. 79:2997-3004 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-79-12-2997 |
| Popis: | IL-2 and equine chorionic gonadotrophin (eCG) initiated reactivation of equid herpesvirus-1 (EHV-1) from venous lymphocytes at a frequency of 1/10(-5). Indirect immunofluorescence showed that > 80% of virus-positive leukocytes were CD5+/CD8+ with the remaining 20% being CD5+/CD8-/CD4-. Cocultivation demonstrated that the reactivated virus was infectious. In addition, virus was reactivated in vitro from leukocytes of > 70% of horses by the mitogens phytohaemagglutinin (PHA) and pokeweed mitogen (PWM). Transfer of supernatants showed that IL-2 and eCG acted indirectly by causing the release of other mediators from adherent cells; these mediators then reactivated EHV-1 from T cells. Blocking experiments with anti-IL-2 showed that PWM and PHA acted via IL-2 but that eCG did not. This is the first clear definition of the lymphoid cells that harbour latent EHV-1 in vivo and correlates with current RT-PCR and in situ hybridization of latency-associated transcripts in lymphocytes. This method of reactivation in vitro can be used to detect horses carrying latent EHV-1 in vivo and also has the potential to dissect the sequence of events involved in reactivation in vitro. |
| Databáze: | OpenAIRE |
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