Continuous Delivery of Stromal Cell-Derived Factor-1 from Alginate Scaffolds Accelerates Wound Healing
| Autor: | Marc S. Penn, Sina Y. Rabbany, Timothy J. Miller, Shahin Rafii, Masaya Yamamoto, Rahul Aras, Joseph Pastore |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Chemokine
Pathology medicine.medical_specialty Stromal cell Alginates Biomedical Engineering lcsh:Medicine Biocompatible Materials Pharmacology Tissue scarring Hydrogel Polyethylene Glycol Dimethacrylate Neovascularization Mice Tissue remodeling Glucuronic Acid In vivo medicine Animals Humans Stromal cell-derived factor 1 Pressure Ulcer Wound Healing Transplantation biology business.industry Hexuronic Acids lcsh:R Hydrogels Surgical wound Cell Biology Chemokine CXCL12 Recombinant Proteins In vitro Kinetics biology.protein medicine.symptom Wound healing business Plasmids Dermal delivery |
| Zdroj: | Cell Transplantation, Vol 19 (2010) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | Proper wound diagnosis and management is an increasingly important clinical challenge and is a large and growing unmet need. Pressure ulcers, hard-to-heal wounds, and problematic surgical incisions are emerging at increasing frequencies. At present, the wound-healing industry is experiencing a paradigm shift towards innovative treatments that exploit nanotechnology, biomaterials, and biologics. Our study utilized an alginate hydrogel patch to deliver stromal cell-derived factor-1 (SDF-1), a naturally occurring chemokine that is rapidly overexpressed in response to tissue injury, to assess the potential effects SDF-1 therapy on wound closure rates and scar formation. Alginate patches were loaded with either purified recombinant human SDF-1 protein or plasmid expressing SDF-1 and the kinetics of SDF-1 release were measured both in vitro and in vivo in mice. Our studies demonstrate that although SDF-1 plasmid- and protein-loaded patches were able to release therapeutic product over hours to days, SDF-1 protein was released faster (in vivo Kd0.55 days) than SDF-1 plasmid (in vivo Kd3.67 days). We hypothesized that chronic SDF-1 delivery would be more effective in accelerating the rate of dermal wound closure in Yorkshire pigs with acute surgical wounds, a model that closely mimics human wound healing. Wounds treated with SDF-1 protein ( n = 10) and plasmid ( n = 6) loaded patches healed faster than sham ( n = 4) or control ( n = 4). At day 9, SDF-1-treated wounds significantly accelerated wound closure (55.0 ± 14.3% healed) compared to nontreated controls (8.2 ± 6.0%, p < 0.05). Furthermore, 38% of SDF-1-treated wounds were fully healed at day 9 (vs. none in controls) with very little evidence of scarring. These data suggest that patch-mediated SDF-1 delivery may ultimately provide a novel therapy for accelerating healing and reducing scarring in clinical wounds. |
| Databáze: | OpenAIRE |
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