Critical evaluation of the use of total reflection X-ray fluorescence spectrometry for the analysis of whole blood samples: application to patients with thyroid gland diseases
Autor: | Ana-Marija Domijan, Suzana Inić, Renato Janušić, Jasna Jablan, Verica Garaj-Vrhovac, Marko Gerić, Ignasi Queralt, Božena Šarčević, Eva Marguí |
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Přispěvatelé: | Queralt, Ignacio [0000-0002-8790-8382], Queralt, Ignacio |
Rok vydání: | 2019 |
Předmět: |
Adult
Accuracy and precision Sample (material) Fluorescence spectrometry Thyroid Gland X-ray fluorescence 02 engineering and technology Mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry Humans Whole blood Aged Whole blood Multielement analysis TXRF Thyroid Detection limit Thyroid Residue (complex analysis) Chromatography Blood -- Analysis Chemistry 010401 analytical chemistry Spectrometry X-Ray Emission Espectroscòpia de raigs X Middle Aged 021001 nanoscience & nanotechnology Elements Thyroid Diseases 0104 chemical sciences X-ray spectroscopy Sang -- Anàlisi TXRF 0210 nano-technology Blood Chemical Analysis Multielement analysis |
Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname © Analytical and Bioanalytical Chemistry, 2019, vol. 411. p. 1659-1670 Articles publicats (D-Q) DUGiDocs – Universitat de Girona |
Popis: | Multielemental analysis of whole blood can provide significant information for the evaluation of nutritional status and diagnosis of certain diseases as well as for the assessment of exposure to potentially toxic metals. However, the quantification of multiple elements in whole blood is not easy partly because of the wide variation in element concentrations (from ng L −1 to g L −1 ) and the complex matrix. The aim of this work was to develop a fast, sustainable, and reliable analytical method, in combination with low-power TXRF, for multielemental analysis of blood samples. Firstly, a set of experiments were carried out to select the best diluent type and dilution factor using the control material SeronormTM Trace Elements Whole Blood L-1. A critical evaluation of the parameters affecting the sample deposition on the reflector was also carried out including a study of the shape and element distribution of the deposited residue on the reflector by micro X-ray fluorescence spectrometry. Using the best analytical conditions, limits of detection estimated were in the low milligrams per kilogram range and similar to those obtained using more complex sample treatments such as digestion. Accuracy and precision of the results were in most cases acceptable (recoveries 89–102%, RSD 6–8%, n = 5). Only underestimated values were obtained for light elements such as potassium. To prove the applicability of the method, several blood samples from control and thyroid disease patients were analyzed. Despite the fact that more samples need to be analyzed, it seems that Zn and Br contents in some of the patients are significantly higher compared to control samples. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. |
Databáze: | OpenAIRE |
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