Immuno-histochemical evaluation of solar lentigines: The association of KGF/KGFR and other factors with lesion development
| Autor: | Dianne Rossetti, Connie B. Lin, Cassarino David, Nannan Chen, Miri Seiberg, Andrzej Slominski, Yaping Hu |
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| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Fibroblast Growth Factor 7 Biopsy Human skin Stem cell factor Dermatology Biology Fibroblast growth factor Stem cell marker Biochemistry Lesion chemistry.chemical_compound Downregulation and upregulation Hyperpigmentation medicine Humans Receptor PAR-2 Receptor Fibroblast Growth Factor Type 2 Molecular Biology Aged Skin Stem Cell Factor integumentary system Keratin-15 Monophenol Monooxygenase Middle Aged Skin Aging chemistry Case-Control Studies Disease Progression Immunohistochemistry Keratinocyte growth factor medicine.symptom |
| Zdroj: | Journal of dermatological science. 59(2) |
| ISSN: | 1873-569X |
| Popis: | Background: Solar lentigines (SLs) are macular hyperpigmented lesions associated with sun exposure and age. Histopathologically, SLs are defined by a hyperpigmented basal layer and elongated rete ridges. The molecular mechanisms involved in the formation and the development of SLs are not completely understood. Our earlier data show that keratinocyte growth factor (KGF) induces hyperpigmentary lesions with histological resemblance to SLs. Objective: To investigate the association of KGF/KGF receptor (KGFR) and other pigmentary genes with the progression of SL development. To better understand the possible role of KGF in the pathology of SLs. Methods: Archived human skin biopsies (24 SLs and 14 healthy skins) were studied using immunohistochemistry for KGF/KGFR, proliferation marker Ki67, stem cell marker keratin-15 (K15), tyrosinase (TYR), stem cell factor (SCF), and protease-activated receptor-2 (PAR-2). Results: An increase in TYR-positive cells and expression was found throughout SL progression, as compared to normal skin. The levels of KGF, KGFR, SCF, Ki67 and PAR-2 varied during SL progression. Ki67, K15 and KGF/KGFR were significantly upregulated at early-mid SL stages. The latest-stage SLs expressed the lowest levels of KGF, KGFR, SCF, Ki67 and PAR-2. Conclusions: The upregulation of KGF/KGFR might induce the formation of rete ridges and hyperpigmentation. The reduced levels of all examined proteins (except TYR and K15) suggest a possible inactive status (dormancy or quiescence) of advanced lesions. |
| Databáze: | OpenAIRE |
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