SDHB and SDHA Immunohistochemistry in Canine Pheochromocytomas
| Autor: | Omar A. Al-Mahmood, Michael J. Dark, Melissa A. Brown, Firas M. Abed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endocrine system SDHB SDHA macromolecular substances SDH Biology Gene mutation medicine.disease_cause Article Pheochromocytoma 03 medical and health sciences 0302 clinical medicine lcsh:Zoology medicine lcsh:QL1-991 Mutation lcsh:Veterinary medicine General Veterinary Succinate dehydrogenase medicine.disease Molecular biology pheochromocytoma Immunohistochemistry 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis dog biology.protein lcsh:SF600-1100 Animal Science and Zoology Adrenal medulla |
| Zdroj: | Animals, Vol 10, Iss 1683, p 1683 (2020) Animals : an Open Access Journal from MDPI Animals Volume 10 Issue 9 |
| ISSN: | 2076-2615 |
| Popis: | Pheochromocytomas (PCs) are tumors arising from the chromaffin cells of the adrenal glands and are the most common tumors of the adrenal medulla in animals. In people, these are highly correlated to inherited gene mutations in the succinate dehydrogenase (SDH) pathway however, to date, little work has been done on the genetic basis of these tumors in animals. In humans, immunohistochemistry has proven valuable as a screening technique for SDH mutations. Human PCs that lack succinate dehydrogenase B (SDHB) immunoreactivity have a high rate of mutation in the SDH family of genes, while human PCs lacking succinate dehydrogenase A (SDHA) immunoreactivity have mutations in the SDHA gene. To determine if these results are similar for dogs, we performed SDHA and SDHB immunohistochemistry on 35 canine formalin-fixed, paraffin-embedded (FFPE) PCs. Interestingly, there was a loss of immunoreactivity for both SDHA and SDHB in four samples (11%), suggesting a mutation in SDHx including SDHA. An additional 25 (71%) lacked immunoreactivity for SDHB, while retaining SDHA immunoreactivity. These data suggest that 29 out of the 35 (82%) may have an SDH family mutation other than SDHA. Further work is needed to determine if canine SDH immunohistochemistry on PCs correlates to genetic mutations that are similar to human PCs. |
| Databáze: | OpenAIRE |
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