Characterization of a Monoclonal Antibody Specific for Novel Bcr/Abl Out-of-Frame Fusion Proteins
| Autor: | Carla Alba Cabras, Katia Crotta, Giuseppe Saglio, Ornella Marelli, Donatella Lattuada, Cristina Panuzzo, C. Casnici, Gisella Volpe, Renato Longhi |
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| Rok vydání: | 2011 |
| Předmět: |
Blotting
Western Immunology Fusion Proteins bcr-abl Fluorescent Antibody Technique Centrifugation Enzyme-Linked Immunosorbent Assay Mice Transgenic Genes abl Biology Philadelphia chromosome Cell Line Mice Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases Acute lymphocytic leukemia medicine Animals Humans Immunoprecipitation Immunology and Allergy Frameshift Mutation neoplasms Chromatography High Pressure Liquid Hybridomas ABL breakpoint cluster region Antibodies Monoclonal Dendritic Cells Precursor Cell Lymphoblastic Leukemia-Lymphoma Flow Cytometry medicine.disease Fusion protein Leukemia Cancer research Chronic myelogenous leukemia K562 cells |
| Zdroj: | Hybridoma (2005) 30 (2011): 261–269. doi:10.1089/hyb.2010.0122 info:cnr-pdr/source/autori:Casnici, Claudia; Volpe, Gisella; Crotta, Katia; Panuzzo, Cristina; Lattuada, Donatella; Cabras, Carla Alba; Longhi, Renato; Saglio, Giuseppe; Marelli, Ornella/titolo:Characterization of a Monoclonal Antibody Specific for Novel Bcr%2FAbl Out-of-Frame Fusion Proteins/doi:10.1089%2Fhyb.2010.0122/rivista:Hybridoma (2005)/anno:2011/pagina_da:261/pagina_a:269/intervallo_pagine:261–269/volume:30 |
| ISSN: | 1557-8348 1554-0014 |
| DOI: | 10.1089/hyb.2010.0122 |
| Popis: | The new tumor-specific antigens Bcr/Abl-OOF, identified in Philadelphia chromosome (Ph)-positive leukemia cells, are derived from an alternative splicing event involving BCR exons 1, 13, or 14 and ABL exons 4 and 5. The COOH-terminus of these transcription products contain an amino acid portion derived from an out-of-frame (OOF) reading of the ABL gene; these variants are expressed in Ph-positive chronic myelogenous leukemia (CML) and acute lymphocytic leukemia patients. Previously, we confirmed the presence of out-of-frame peptide-specific T cells in the peripheral blood of CML patients with the ability to lyse primary autologous CML cells. We also demonstrated that the out-of-frame Abl portion was immunogenic in HLA-A2.1 transgenic mice. Here we describe the production and characterization of monoclonal antibody 1D8G8, a new tool for localization and functional studies of the tumor antigen Bcr/Abl-OOF. This antibody recognizes the out-of-frame protein portion of the native full-length Bcr/Abl-OOF protein expressed in cells transiently transfected, as demonstrated by immunoprecipitation and immunofluorescence, and binds to a specific epitope of this antigen presented in association with HLA-A2.1 molecules at the surface of these cells, as demonstrated by flow cytometry. Thus this MAb could be useful to better understand how this new protein presents in Ph-positive cells beside the canonical Bcr/Abl fusion proteins. |
| Databáze: | OpenAIRE |
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