Biological Validation of a Chemical Effluent Decontamination System
| Autor: | David E. Harbourt, Jennifer C. Hoover, Ashley E. Piper, T.L. Buhr, Pamela J. Glass, Joel A. Bozue, Christopher P. Klimko, Jeremy A. Miller, Melissa G. Hunter, Jessica M. Weidner, Jennifer L. Shoe, Brian R. Sauerbry, Christopher K. Cote |
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| Rok vydání: | 2021 |
| Předmět: |
Bacillus (shape)
0303 health sciences Bleach biology Waste management 030306 microbiology Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Original Articles Human decontamination Management Monitoring Policy and Law biology.organism_classification 03 medical and health sciences Biological validation Environmental science Effluent 030304 developmental biology Biotechnology |
| Zdroj: | Appl Biosaf |
| ISSN: | 2470-1246 1535-6760 |
| Popis: | Introduction: Failure of an existing effluent decontamination system (EDS) prompted the consideration of commercial off-the-shelf solutions for decontamination of containment laboratory waste. A bleach-based chemical EDS was purchased to serve as an interim solution. Methods: Studies were conducted in the laboratory to validate inactivation of Bacillus spores with bleach in complex matrices containing organic simulants including fetal bovine serum, humic acid, and animal room sanitation effluent. Results: These studies demonstrated effective decontamination of >106 spores at a free chlorine concentration of ≥5700 parts per million with a 2-hour contact time. Translation of these results to biological validation of the bleach-based chemical EDS required some modifications to the system and its operation. Discussion: The chemical EDS was validated for the treatment of biosafety levels 3 and 4 waste effluent using laboratory-prepared spore packets along with commercial biological indicators; however, several issues and lessons learned identified during the process of onboarding are also discussed, including bleach product source, method of validation, dechlorination, and treated waste disposal. |
| Databáze: | OpenAIRE |
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