Autochthonous microalgae cultivation with anaerobic effluent: isolation of strains, survivorship, and characterization of the produced biomass
| Autor: | Aline Figueredo Dassoler, Helenice Silva de Jesus, Sérvio Túlio Alves Cassini, Marcos Vinicius Pereira, Ricardo Franci Gonçalves |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
020209 energy
02 engineering and technology Chlorophyta 010501 environmental sciences Aquatic Science sobrevivência 01 natural sciences anaerobic effluent Nutrient Chlorococcum 0202 electrical engineering electronic engineering information engineering efluente anaeróbio Effluent Scenedesmus lcsh:Environmental sciences 0105 earth and related environmental sciences General Environmental Science lcsh:GE1-350 biology Chemistry microalgae Public Health Environmental and Occupational Health survivorship Desmodesmus biology.organism_classification Horticulture microalgas Wastewater Coccomyxa |
| Zdroj: | Revista Ambiente & Água, Vol 14, Iss 4, Pp 1-9 (2019) Revista Ambiente & Água v.14 n.4 2019 Revista Ambiente & Água Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) instacron:IPABHI Revista Ambiente & Água, Volume: 14, Issue: 4, Article number: e2362, Published: 18 JUL 2019 |
| Popis: | Six Chlorophyta strains were isolated from the effluent of an anaerobic reactor treating municipal wastewater and identified as Desmodesmus sp. L02, Chlorococcum sp. L04, Coccomyxa sp. L05, Chlorella sp. L06, Scenedesmus sp. L08 and Tetradesmus sp. L09. The microalgae strains were cultivated in unsterilized wastewater under laboratory conditions to determine their potential to survive under non-sterile conditions. The strains were also cultivated in sterilized wastewater in order to analyze their nutrient removal potential and characterize the produced biomass. Amongst the isolated microalgae, Chlorella sp. L06 had the highest survivorship percentage (90%) for ten days of culture, whilst Desmodesmus sp. L02 had the lowest, not exceeding 1.8% after 24h of inoculation. The dried biomass of the isolates showed an average of 28.7% of protein, 15.4% of lipids and 14.8% of carbohydrates, with Chlorococcum sp. L04 reaching 29.3% of carbohydrates. In terms of nutrients, nitrogen removal varied from 59.2 to 93%, and phosphorus removal ranged from 79.1 to 95.4%, with Tetradesmus sp. L09 being the most efficient strain. Resumo Seis cepas de Chlorophyta foram isoladas do efluente de um reator anaeróbio tratando esgoto sanitário e identificadas como Desmodesmus sp. L02, Chlorococcum sp. L04, Coccomyxa sp. L05, Chlorella sp. L06, Scenedesmus sp. L08 e Tetradesmus sp. L09. As cepas de microalgas foram cultivadas em efluente não-esterilizado em condições de laboratório para determinar os potenciais de sobrevivência sob condições não estéreis. As cepas também foram cultivadas em efluente esterilizado para avaliar os potenciais de remoção de nutrientes e caracterizar bioquimicamente a biomassa produzida. Entre as microalgas isoladas, Chlorella sp. L06 teve a maior porcentagem de sobrevivência (90%) por dez dias de cultura, enquanto Desmodesmus sp. L02 apresentou o menor valor, não excedendo 1,8% após 24h de inoculação. A biomassa seca dos isolados apresentou uma média de 28,7% de proteína, 15,4% de lipídios e 14,8% de carboidratos, com Chlorococcum sp. L04 atingindo 29,3% de carboidratos. Em termos de nutrientes, a remoção de nitrogênio variou de 59,2 a 93%, e a remoção de fósforo variou de 79,1 a 95,4%, com Tetradesmus sp. L09 sendo a cepa mais eficiente. |
| Databáze: | OpenAIRE |
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