Discovery and validation of quality markers of Fructus Aurantii against acetylcholinesterase using metabolomics and bioactivity assays
| Autor: | Xiao-Fang Ma, Shou-Wen Zhang, Yi-Kun Wang, Zi-Meng Zhou, Man-Yun Dai, Hong-Ning Liu, Fei Li, Xue-Rong Xiao |
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| Rok vydání: | 2021 |
| Předmět: |
Male
China Citrus Filtration and Separation Biology 01 natural sciences Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Chemical marker Coumarins Drug Discovery Animals Statistical analysis Naringin Inhibitory effect Neohesperidin Traditional medicine Serum pharmacochemistry Hesperidin 010401 analytical chemistry Acetylcholinesterase 0104 chemical sciences Rats chemistry 030220 oncology & carcinogenesis Flavanones Cholinesterase Inhibitors Biomarkers |
| Zdroj: | Journal of separation scienceREFERENCES. 44(11) |
| ISSN: | 1615-9314 |
| Popis: | Fructus Aurantii is a traditional medicated diet in East Asia. To determine the underlying chemical markers responsible for the quality and efficacy of Fructus Aurantii, a sensitive metabolomic method was applied to distinguish Fructus Aurantii in Jiangxi Province from other two geographical locations (Hunan Province and Chongqi City) in China. In the present study, multivariate analyses were adopted to compare chemical compositions in 21 batches of Fructus Aurantii samples. Among three geographical origins, 23 differential compounds were structurally identified. Serum pharmacochemistry exhibited that 22 components could be detected in rat serum. Six differential and absorbed components were selected as six potential markers. Statistical analysis of the effects of six potential markers on the quality of Fructus Aurantii revealed that the content of markers varied widely in three origins. Six differential and absorbed components were evaluated further biological activity. Neohesperidin, naringin and meranzin showed inhibitory effect on acetylcholinesterase that regulates gastrointestinal motility in vitro and in silico, suggesting that these three components may be determined as the active biomarkers of Fructus Aurantii. These findings demonstrate the potential of biomarkers for identification and quality control of Fructus Aurantii. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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