Measuring Real-time DNA/RNA Nuclease Activity through Fluorescence
| Autor: | Sally L. Rogers, Paulina Wyrzykowska, Richard Chahwan |
|---|---|
| Přispěvatelé: | University of Zurich, Chahwan, Richard |
| Rok vydání: | 2021 |
| Předmět: |
DNA repair
Strategy and Management 610 Medicine & health 10263 Institute of Experimental Immunology Industrial and Manufacturing Engineering Cofactor chemistry.chemical_compound 1300 General Biochemistry Genetics and Molecular Biology 2400 General Immunology and Microbiology 1110 Plant Science Methods Article Ribonuclease UFSP13-5 Translational Cancer Research chemistry.chemical_classification Nuclease biology Mechanical Engineering Metals and Alloys 2800 General Neuroscience RNA Fluorescence Enzyme Biochemistry chemistry biology.protein 570 Life sciences DNA |
| Zdroj: | Bio Protoc |
| DOI: | 10.5167/uzh-231133 |
| Popis: | DNA and RNA nucleases are wide-ranging enzymes, taking part in broad cellular processes from DNA repair to immune response control. Growing interest in the mechanisms and activities of newly discovered nucleases inspired us to share the detailed protocol of our nuclease assay ( Sheppard et al., 2019 ). This easy and inexpensive method can provide data that enables understanding of the molecular mechanism for novel or tested nucleases, from substrate preference and cofactors involved to catalytic rate of reaction. |
| Databáze: | OpenAIRE |
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