Discovery and Engineering of an Aldehyde Tolerant 2-deoxy-D-ribose 5-phosphate Aldolase (DERA) from Pectobacterium atrosepticum
Autor: | Meera Haridas, Carolin Bisterfeld, Le Min Chen, Stefan R. Marsden, Fabio Tonin, Rosario Médici, Adolfo Iribarren, Elizabeth Lewkowicz, Peter-Leon Hagedoorn, Ulf Hanefeld, Eman Abdelraheem |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
acetaldehyde resistance
Mutant DERA medicine.disease_cause lcsh:Chemical technology 01 natural sciences Aldehyde Catalysis lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound aldolase medicine lcsh:TP1-1185 Physical and Theoretical Chemistry Pectobacterium atrosepticum Escherichia coli 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 010405 organic chemistry Aldolase A Acetaldehyde 0104 chemical sciences Enzyme Biochemistry chemistry lcsh:QD1-999 biology.protein Specific activity |
Zdroj: | Catalysts, Vol 10, Iss 883, p 883 (2020) Catalysts Volume 10 Issue 8 Catalysts, 10(8) |
ISSN: | 2073-4344 |
Popis: | DERA (2-Deoxy-D-ribose 5-phosphate aldolase) is the only known aldolase that accepts two aldehyde substrates, which makes it an attractive catalyst for the synthesis of a chiral polyol motif that is present in several pharmaceuticals, such as atorvastatin and pravastatin. However, inactivation of the enzyme in the presence of aldehydes hinders its practical application. Whole cells of Pectobacterium atrosepticum were reported to exhibit good tolerance toward acetaldehyde and to afford 2-deoxyribose 5-phosphate with good yields. The DERA gene (PaDERA) was identified, and both the wild-type and a C49M mutant were heterologously expressed in Escherichia coli. The purification protocol was optimized and an initial biochemical characterization was conducted. Unlike other DERAs, which show a maximal activity between pH 4.0 and 7.5, PaDERA presented an optimum pH in the alkaline range between 8.0 and 9.0. This could warrant its use for specific syntheses in the future. PaDERA also displayed fourfold higher specific activity than DERA from E. coli (EcDERA) and displayed a promising acetaldehyde resistance outside the whole-cell environment. The C49M mutation, which was previously identified to increase acetaldehyde tolerance in EcDERA, also led to significant improvements in the acetaldehyde tolerance of PaDERA. |
Databáze: | OpenAIRE |
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