High incidence of the t(2;5)(p23;q35) translocation in anaplastic large cell lymphoma and its lack of detection in Hodgkin's disease. Comparison of cytogenetic analysis, reverse transcriptase-polymerase chain reaction, and P-80 immunostaining

Autor: Laurence Lamant, M Shiuta, Georges Delsol, T al Saati, S Mori, BB de Paillerets, Fabienne Meggetto, H. Rubie, Alain Bernheim, Nicole Dastugue, A. Robert, D Schlaifer, F Rigal, M J Terrier-Lacombe, Laurence Brugières
Rok vydání: 1996
Předmět:
Pathology
Biopsy
Chromosomal translocation
Polymerase Chain Reaction
Biochemistry
Translocation
Genetic
Immunoenzyme Techniques
hemic and lymphatic diseases
Tumor Cells
Cultured
Anaplastic lymphoma kinase
Anaplastic Lymphoma Kinase
Reed-Sternberg Cells
Child
Anaplastic large-cell lymphoma
Aged
80 and over
Age Factors
Antibodies
Monoclonal
DNA
Neoplasm
Hematology
Middle Aged
Protein-Tyrosine Kinases
Hodgkin Disease
Neoplasm Proteins
Chromosomes
Human
Pair 1
Child
Preschool
Chromosomes
Human
Pair 2
Chromosomes
Human
Pair 5
Lymphoma
Large-Cell
Anaplastic
Immunohistochemistry
Antibody
Adult
medicine.medical_specialty
Adolescent
Molecular Sequence Data
Immunology
Biology
Sensitivity and Specificity
Biomarkers
Tumor
medicine
Humans
Aged
Base Sequence
Large cell
Receptor Protein-Tyrosine Kinases
Cell Biology
medicine.disease
Molecular biology
Lymphoma
Karyotyping
biology.protein
Immunostaining
Zdroj: Blood. 87:284-291
ISSN: 1528-0020
0006-4971
DOI: 10.1182/blood.v87.1.284.284
Popis: Fifty-six cases of anaplastic large cell lymphoma (ALCL), 23 cases of Hodgkin's disease, and 16 cases of diffuse large cell lymphoma were investigated for the t(2;5)(p23;q35) translocation. The translocation was detected by using cytogenetic analysis, reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry with P80 antibody directed against the kinase domain of anaplastic lymphoma kinase (ALK) of the chimeric NPM/ALK protein. In all but three cases of ALCL, we found an agreement between cytogenetic analysis, RT-PCR, and P80 staining. However, in one case, the t(2;5) translocation was detected with cytogenetic analysis, but RT-PCR and P80 staining were found to be negative. Conversely, in another case the karyotype was normal, but the hybrid mRNA and P80 staining were found to be positive. In one case, malignant cells showed a translocation involving chromosomes 1q25 and 2p23 and were strongly positive for P80 staining. Such a result could be expected because P80 antibody detects the kinase domaine of the ALK protein encoded by chromosome 2p23. Overall 73.2% (41 of 56) of cases were found to be positive. However, the highest percentage (23 of 26 cases; 88.5%) of P80 positive cases was found in children compared with 60% (18 of 30 cases) in adult ALCL (P < .05). In Hodgkin's disease, Reed-Sternberg cells were found to be clearly negative by RT-PCR and with P80 antibody. The latter results suggest that Hodgkin's disease and t(2;5)-positive ALCL are distinct biological entities and that the demonstration of the t(2;5) translocation is of diagnostic importance in differentiating these two entities. The results of the present study indicate that immunohistochemistry with P80 antibody is a reliable method for detecting NPM/ALK chimeric protein.
Databáze: OpenAIRE
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