Testicular human spermatozoa cryopreservation correlation between sperm head vacuoles, DNA fragmentation and mitochondrial membrane potential
| Autor: | Morteza Anvari, Mohammad Ali Khalili, Sahabeh Etebary, Nahid Yari, Seyed Mehdi Kalantar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Membrane potential endocrine system lcsh:R5-920 030219 obstetrics & reproductive medicine lcsh:QH471-489 urogenital system Obstetrics and Gynecology Vacuole Biology Sperm Cryopreservation Testicular sperm extraction Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Reproductive Medicine Vacuolization Organelle DNA fragmentation lcsh:Reproduction lcsh:Medicine (General) reproductive and urinary physiology |
| Zdroj: | Middle East Fertility Society Journal, Vol 23, Iss 4, Pp 413-417 (2018) |
| ISSN: | 1110-5690 |
| Popis: | Background: There are difficulties associated with testicular sperm freezing. Different methods of sperm cryopreservation developed. Not enough detailed studies about the real efficacy of these techniques exist. For sperm morphologic assessment, motile sperm organelle morphology examination (MSOME) is able to identify not only conventional morphological sperm alterations but also sperm head vacuoles. Objective: To assess the effect of cryopreservation on testicular spermatozoa vacuoles by MSOME and its correlation to DNA fragmentation and mitochondrial membrane potential. Materials and methods: After preparation, testicular sperm extraction samples of 15 azoospermic men, aged 20–40 years old were divided into three groups. Group 1 was assessed freshly. Group 2 was cryopreserved with vitrification method and Group 3 with cooling in liquid nitrogen vapor using droplet. Pre and post warming assessment in terms of spermatozoa head vacuoles by MSOME, DNA fragmentation, and mitochondrial membrane potential were performed. Results: The number of spermatozoa with no vacuoles significantly decreased after two cryopreservation techniques (P 0.05). DNA fragmentation and mitochondrial membrane potential increased after cryopreservation (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|