Accuracy of PCR targeting different markers for Staphylococcus aureus identification: a comparative study using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as the gold standard
| Autor: | Wondwossen A. Gebreyes, Mauro M.S. Saraiva, Lauro Santos Filho, Candice M C G de Leon, Danilo Tancler Stipp, Celso J Oliveira, Silvana C Santos, Miliane M Souza |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Methicillin-Resistant Staphylococcus aureus Staphylococcus aureus 030106 microbiology Pcr assay Matrix assisted laser desorption ionization time of flight Biology medicine.disease_cause Mass spectrometry Polymerase Chain Reaction Sensitivity and Specificity 03 medical and health sciences fluids and secretions medicine Animals Humans Pathogen General Veterinary Gold standard (test) Staphylococcal Infections Molecular biology 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Fast methods Brief Communications Staphylococcus |
| Popis: | Staphylococcus aureus is considered a major pathogen in veterinary and human medicine, and the emergence of multidrug-resistant strains, such as livestock-associated methicillin-resistant S. aureus, means that reliable, inexpensive, and fast methods are required to identify S. aureus obtained from animal sources. We tested the accuracy of a PCR targeting the genes femA, nuc, and coa in identifying S. aureus from animals. A total of 157 Staphylococcus spp. isolates were examined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry; 18 different Staphylococcus species were identified. Of 68 S. aureus isolates, the genes femA, nuc, and coa were found in 61, 53, and 32 isolates, respectively. Considering MALDI-TOF as the gold standard, the PCR assays targeting all 3 genes showed 100% specificity; the sensitivity values were 89.7, 77.9, and 47.0% for femA, nuc, and coa, respectively. Sensitivity was 100% when femA and nuc markers were targeted simultaneously. These results confirm PCR as an accurate method to identify S. aureus species from animal sources and strongly suggest the simultaneous use of primers targeting femA and nuc genes. |
| Databáze: | OpenAIRE |
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