A durable murine model of spleen transplantation with arterial and venous anastomoses
| Autor: | Ralph Weissleder, José Luiz Figueiredo, José L. Lima-Filho, Fernando Santa-Cruz, Ingo Hilgendorf, Masanori Aikawa, Matthias Nahrendorf, Filip K. Swirski, Mikael J. Pittet, Clinton S. Robbins |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty medicine.medical_treatment Congenic lcsh:Medicine Spleen 030204 cardiovascular system & hematology Immunofluorescence Article Flow cytometry Veins 03 medical and health sciences Mice 0302 clinical medicine Immune system medicine Animals lcsh:Science Transplantation Multidisciplinary medicine.diagnostic_test business.industry Organ transplantation Spleen transplantation lcsh:R Anastomosis Surgical Graft Survival Blood flow Arteries 3. Good health Experimental models of disease 030104 developmental biology medicine.anatomical_structure Lymphatic system Models Animal lcsh:Q business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-7 (2020) |
| ISSN: | 2045-2322 |
| Popis: | The spleen is a large lymphoid organ located in the abdomen that filters blood and regulates the immune system. The extent of mobilization of splenic immune cells to peripheral tissues in health and disease, however, remains poorly understood. This is due, in large part, to a lack of in vivo, spleen-specific lineage tagging strategies. Here, we describe a detailed practical protocol of spleen transplantation and its evaluation for long-term graft survival. Unlike implantation of splenic morsels in the great omentum, our approach uses arterial and venous anastomoses which rapidly restores blood flow and facilitates long-term survival of the graft. The use of congenic mouse strains permits the use of immunofluorescence and flow cytometry-based methodologies to unambiguously track the migration of spleen-derived cells to peripheral tissues. |
| Databáze: | OpenAIRE |
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