The ‘obligate diploid’ Candida albicans forms mating-competent haploids
| Autor: | Ching Hua Su, Benjamin D. Harrison, Anja Forche, Meleah A. Hickman, Darren Abbey, Matthew P. Hirakawa, Guisheng Zeng, Yan-Ming Wang, Richard J. Bennett, Yue Wang, Judith Berman |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Heterozygote Mating type Cell Separation Haploidy Biology Article Loss of heterozygosity Mice 03 medical and health sciences Stress Physiological Candida albicans Animals Serial Passage Mating 030304 developmental biology Genetics Mice Inbred ICR 0303 health sciences Multidisciplinary Virulence Obligate 030306 microbiology Homozygote fungi Chlamydospore formation Flow Cytometry biology.organism_classification Diploidy Genetic Techniques Haplotypes Mating of yeast Sex Genetic Fitness Ploidy Gene Deletion |
| Zdroj: | Nature. 494:55-59 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/nature11865 |
| Popis: | Candida albicans, the most prevalent human fungal pathogen, is considered to be an obligate diploid that carries recessive lethal mutations throughout the genome. Here we demonstrate that C. albicans has a viable haploid state that can be derived from diploid cells under in vitro and in vivo conditions, and that seems to arise through a concerted chromosome loss mechanism. Haploids undergo morphogenetic changes like those of diploids, including the yeast-hyphal transition, chlamydospore formation and a white-opaque switch that facilitates mating. Haploid opaque cells of opposite mating type mate efficiently to regenerate the diploid form, restoring heterozygosity and fitness. Homozygous diploids arise spontaneously by auto-diploidization, and both haploids and auto-diploids show a similar reduction in fitness, in vitro and in vivo, relative to heterozygous diploids, indicating that homozygous cell types are transient in mixed populations. Finally, we constructed stable haploid strains with multiple auxotrophies that will facilitate molecular and genetic analyses of this important pathogen. |
| Databáze: | OpenAIRE |
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