Multiplex bioimaging of proteins-related to neurodegenerative diseases in eye sections by laser ablation - inductively coupled plasma – mass spectrometry using metal nanoclusters as labels
| Autor: | Ana Lores-Padín, Lydia Álvarez, Rosario Pereiro, Beatriz Fernández, Imre Lengyel, Héctor González-Iglesias |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Resolution (mass spectrometry)
Multiplex analysis of proteins 02 engineering and technology Eye Mass spectrometry 01 natural sciences Mass Spectrometry Analytical Chemistry Nanoclusters Ciliary body SDG 3 - Good Health and Well-being medicine Humans Eye Proteins Retina Retinal pigment epithelium Laser ablation ICP-MS Metal nanoclusters biology Chemistry Age-related macular degeneration 010401 analytical chemistry Neurodegenerative Diseases 021001 nanoscience & nanotechnology Bioimaging Immunohistochemistry Primary and secondary antibodies eye diseases 0104 chemical sciences medicine.anatomical_structure Metals biology.protein Biophysics Metallothionein Laser Therapy sense organs Inductively coupled plasma 0210 nano-technology |
| Zdroj: | Scopus Lores-Padín, A, Fernández, B, Álvarez, L, González-Iglesias, H, Lengyel, I & Pereiro, R 2021, ' Multiplex bioimaging of proteins-related to neurodegenerative diseases in eye sections by laser ablation-Inductively coupled plasma – Mass spectrometry using metal nanoclusters as labels ', Talanta, vol. 221, 121489 . https://doi.org/10.1016/j.talanta.2020.121489 RUO. Repositorio Institucional de la Universidad de Oviedo instname |
| DOI: | 10.1016/j.talanta.2020.121489 |
| Popis: | Simultaneous determination of proteins with micrometric resolution is a significant challenge. In this study, laser ablation (LA) inductively coupled plasma - mass spectrometry (ICP-MS) was employed to quantify the distribution of proteins associated to the eye disease age-related macular degeneration (AMD) using antibodies labelled with three different metal nanoclusters (MNCs). PtNCs, AuNCs and AgNCs contain hundreds of metal atoms and were used to detect metallothionein 1/2 (MT1/2), complement factor H (CFH) and amyloid precursor protein (APP) in retina, ciliary body, retinal pigment epithelium (RPE), choroid and sclera from human cadaveric eye sections. First, the labelling of MNCs bioconjugated primary antibodies (Ab) was optimised following an immunolabelling protocol to avoid the non-specific interaction of MNCs with the tissue. Then, the LA and ICP-MS conditions were studied to obtain high-resolution images for the simultaneous detection of the three labels at the same tissue section. A significant signal amplification was found when using AuNCs, AgNCs and PtNCs labelled Ab of 310, 723 and 1194 respectively. After the characterisation of MNCs labelled immunoprobes, the Ab labelling was used for determination of MT1/2, CFH and APP in the RPE-choroid-sclera, where accumulation of extracellular deposits related to AMD was observed. Experimental results suggest that this method is fully suitable for the simultaneous detection of at least three different proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|