Scavenging of Dickkopf-1 by macromer-based biomaterials covalently decorated with sulfated hyaluronan displays pro-osteogenic effects
| Autor: | F. Mitrach, Vera Hintze, Matthias Schnabelrauch, Juliane Salbach-Hirsch, Michael C. Hacker, Benno Müller, Mathis Gronbach, V. Lidzba, Lorenz C. Hofbauer, Stephanie Möller, Sandra Rother, Michaela Schulz-Siegmund |
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| Rok vydání: | 2020 |
| Předmět: |
Stromal cell
0206 medical engineering Biomedical Engineering Context (language use) Biocompatible Materials 02 engineering and technology Biochemistry Biomaterials Tissue engineering Osteogenesis medicine Surface plasmon resonance Hyaluronic Acid Bone regeneration Molecular Biology Chemistry Sulfates Biomaterial Cell Differentiation General Medicine 021001 nanoscience & nanotechnology 020601 biomedical engineering medicine.anatomical_structure Biophysics Surface modification Bone marrow 0210 nano-technology Biotechnology |
| Zdroj: | Acta biomaterialia. 114 |
| ISSN: | 1878-7568 |
| Popis: | Dickkopf-1 (DKK1), a Wnt inhibitor secreted by bone marrow stromal cells (MSC), is known to play an important role in long-term non-union bone fracture defects and glucocorticoid induced osteoporosis. Mitigating its effects in early bone defects could improve osteogenesis and bone defect healing. Here, we applied a biomaterial strategy to deplete a defect environment from DKK1 by scavenging the protein via a macromer-based biomaterial covalently decorated with sulfated hyaluronan (sHA3). The material consisted of cross-copolymerized three-armed macromers with a small anchor molecule. Using the glycidyl anchor, polyetheramine (ED900) could be grafted to the material to which sHA3 was efficiently coupled in a separate step. For thorough investigation of material modification, flat material surfaces were generated by fabricating them on glass discs. The binding capability of sHA3 for DKK1 was demonstrated in this study by surface plasmon resonance measurements. Furthermore, the surfaces demonstrated the ability to scavenge and inactivate pathologic amounts of DKK1 from complex media. In a combinatory approach with Wnt3a, we were able to demonstrate that DKK1 is the preferred binding partner of our sHA3-functionalized surfaces. We validated our findings in a complex in vitro setting of differentiating SaOS-2 cells and primary hMSC. Here, endogenous DKK-1 was scavenged resulting in increased osteogenic differentiation indicating that this is a consistent biological effect irrespective of the model system used. Our study provides insights in the mechanisms and efficiency of sHA3 surface functionalization for DKK1 scavenging, which may be used in a clinical context in the future. |
| Databáze: | OpenAIRE |
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