Imaging of VSOP labeled stem cells in agarose phantoms with susceptibility weighted and T2* weighted MR Imaging at 3T: determination of the detection limit
| Autor: | Donald Lobsien, Antje Y. Dreyer, Albrecht Stroh, Karl-Titus Hoffmann, Johannes Boltze |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_treatment
Animal Types lcsh:Medicine Large Animals Signal Ferric Compounds Diagnostic Radiology chemistry.chemical_compound Model Organisms Limit of Detection Molecular Cell Biology medicine Animals Particle Size lcsh:Science Biology Detection limit Multidisciplinary Sheep medicine.diagnostic_test Staining and Labeling business.industry Chemistry Phantoms Imaging Sepharose Stem Cells lcsh:R Magnetic resonance imaging Mesenchymal Stem Cells Stem-cell therapy VSOP Animal Models Magnetic Resonance Imaging Stroke Disease Models Animal Agarose Medicine lcsh:Q Veterinary Science Stem cell Cellular Types T2 weighted Nuclear medicine business Radiology Biomedical engineering Stem Cell Transplantation Research Article Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 5, p e62644 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Objectives This study aimed to evaluate the detectability of stem cells labeled with very small iron oxide particles (VSOP) at 3T with susceptibility weighted (SWI) and T2* weighted imaging as a methodological basis for subsequent examinations in a large animal stroke model (sheep). Materials and Methods We examined ovine mesenchymal stem cells labeled with VSOP in agarose layer phantoms. The experiments were performed in 2 different groups, with quantities of 0–100,000 labeled cells per layer. 15 different SWI- and T2*-weighted sequences and 3 RF coils were used. All measurements were carried out on a clinical 3T MRI. Images of Group A were analyzed by four radiologists blinded for the number of cells, and rated for detectability according to a four-step scale. Images of Group B were subject to a ROI-based analysis of signal intensities. Signal deviations of more than the 0.95 confidence interval in cell containing layers as compared to the mean of the signal intensity of non cell bearing layers were considered significant. Results Group A: 500 or more labeled cells were judged as confidently visible when examined with a SWI-sequence with 0.15 mm slice thickness. Group B: 500 or more labeled cells showed a significant signal reduction in SWI sequences with a slice thickness of 0.25 mm. Slice thickness and cell number per layer had a significant influence on the amount of detected signal reduction. Conclusion 500 VSOP labeled stem cells could be detected with SWI imaging at 3 Tesla using an experimental design suitable for large animal models. |
| Databáze: | OpenAIRE |
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