Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin
| Autor: | Bianca Furdui, Cheikna Zongo, Farid Baba-Moussa, Sorin Marius Avamescu, Ioana Otilia Ghinea, Durand Dah-Nouvlessounon, Michaelle Chokki, Rodica Mihaela Dinica, Mihaela Cudalbeanu, Aly Savadogo, Lamine Baba-Moussa, Robert Raclea |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Health (social science)
antioxidant plant extracts DPPH Plant Science lcsh:Chemical technology Health Professions (miscellaneous) Microbiology β-Carotene–linoleate bleaching 03 medical and health sciences Rutin chemistry.chemical_compound 0302 clinical medicine Chlorogenic acid Benin lcsh:TP1-1185 Naringin 030304 developmental biology 0303 health sciences Momordica biology Traditional medicine Daidzein biology.organism_classification chemistry Polyphenol 030220 oncology & carcinogenesis diabetes mellitus HPLC Quercetin Food Science |
| Zdroj: | Foods, Vol 9, Iss 434, p 434 (2020) Foods Volume 9 Issue 4 |
| ISSN: | 2304-8158 |
| Popis: | Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and &beta carotene-linoleate bleaching assay. The &alpha amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while &beta glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-&beta D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good &alpha amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of &beta glucosidase activity (82.11. ± 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases. |
| Databáze: | OpenAIRE |
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