Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming
| Autor: | Manel Lopez-Bejar, Marc Yeste, Tania García-Martínez, Iris Martínez-Rodero, Teresa Mogas, Erika Alina Ordóñez-León, Manuel Alvarez-Rodriguez, Meritxell Vendrell-Flotats |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Mitochondrial ROS GPX1 Hot Temperature Bestiar boví -- Cria i millora cow cryotop Apoptosis medicine.disease_cause Embryo development cryopreservation Microtubules Antioxidants Cryopreservation lcsh:Chemistry Oogenesis 0302 clinical medicine Cattle -- Fertility Vitrification lcsh:QH301-705.5 Cells Cultured Spectroscopy chemistry.chemical_classification reactive oxygen species 030219 obstetrics & reproductive medicine Gap Junctions Embryo General Medicine Bestiar boví -- Recursos germoplasmàtics -- Crioconservació Glutathione Computer Science Applications Mitochondria mitochondria embryonic structures Female Cattle -- Germplasm resources -- Cryopreservation Aquaporins Article Catalysis Inorganic Chemistry Andrology 03 medical and health sciences spindle configuration Spindle configuration medicine Animals Physical and Theoretical Chemistry Molecular Biology Cryotop Reactive oxygen species Oòcits -- Criopreservació Organic Chemistry Cow embryo development In Vitro Oocyte Maturation Techniques In vitro maturation Oxidative Stress 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 Bestiar boví -- Fecunditat Oocytes gene expression Cattle Gene expression Oxidative stress |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 7547, p 7547 (2020) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona International Journal of Molecular Sciences, 2020, vol. 21, núm. 20, p. 7547 Articles publicats (D-B) García Martínez, Tania Vendrell Flotats, Meritxell Martínez Rodero, Iris Ordóñez León, Erika Alina Álvarez Rodríguez, Manuel López Béjar, Manel Yeste Oliveras, Marc Mogas, Teresa 2020 Glutathione ethyl ester protects in vitro-maturing bovine oocytes against oxidative stress induced by subsequent vitrification/warming International Journal of Molecular Sciences 21 20 7547 DUGiDocs – Universitat de Girona instname International Journal of Molecular Sciences Volume 21 Issue 20 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-&tau ) and gap junctions (CX43) in oocytes and their derived blastocysts. Vitrification gave rise to abnormal spindle microtubule configurations and elevated ROS levels. Supplementation of IVM medium with GSH-OEt before vitrification preserved mitochondrial distribution pattern and diminished both cytoplasmic and mitochondrial ROS contents and percentages of embryos developing beyond the 8-cell stage were similar to those recorded in fresh non-vitrified oocytes. Although not significantly different from control vitrified oocytes, vitrified oocytes after GSH-OEt treatment gave rise to similar day 8-blastocyst and hatching rates to fresh non-vitrified oocytes. No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of GPX1, AQP3 and CX43 in derived blastocysts. The addition of GSH-OEt to the IVM medium before vitrification may be beneficial for embryo development presumably as the consequence of additional anti-oxidant protection during IVM. |
| Databáze: | OpenAIRE |
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