Evaluation of inflammatory skin infiltrate followingAedes aegyptibites in sensitized and non‐sensitized mice reveals saliva‐dependent and immune‐dependent phenotypes
| Autor: | Denise Morais da Fonseca, Josiane B Assis, Anderson Sá-Nunes, Leila S. Neto, Michele S. Barros, Maressa O. Henrique, Margareth Lara Capurro, Ana Paula Lepique |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Saliva Immunology Inflammation Aedes aegypti SALIVA 03 medical and health sciences 0302 clinical medicine Immune system Aedes parasitic diseases Leukocytes medicine Animals Edema Immunology and Allergy Mast Cells Interleukin 5 Sensitization Skin Mice Inbred BALB C Salivary gland biology Insect Bites and Stings Original Articles biology.organism_classification Cellular Infiltrate Chemotaxis Leukocyte Disease Models Animal Kinetics 030104 developmental biology medicine.anatomical_structure Cellular Microenvironment Neutrophil Infiltration Female medicine.symptom 030215 immunology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Immunology |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | During probing and blood feeding, haematophagous mosquitoes inoculate a mixture of salivary molecules into their vertebrate hosts’ skin. In addition to the anti‐haemostatic and immunomodulatory activities, mosquito saliva also triggers acute inflammatory reactions, especially in sensitized hosts. Here, we characterize the oedema and the cellular infiltrate following Aedes aegypti mosquito bites in the skin of sensitized and non‐sensitized BALB/c mice by flow cytometry. Ae. aegypti bites induced an increased oedema in the ears of both non‐sensitized and salivary gland extract‐ (SGE‐)sensitized mice, peaking at 6 hr and 24 hr after exposure, respectively. The quantification of the total cell number in the ears revealed that the cellular recruitment was more robust in SGE‐sensitized mice than in non‐sensitized mice, and the histological evaluation confirmed these findings. The immunophenotyping performed by flow cytometry revealed that mosquito bites were able to produce complex changes in cell populations present in the ears of non‐sensitized and SGE‐sensitized mice. When compared with steady‐state ears, the leucocyte populations significantly recruited to the skin after mosquito bites in non‐sensitized and sensitized mice were eosinophils, neutrophils, monocytes, inflammatory monocytes, mast cells, B‐cells and CD4(+) T‐cells, each one with its specific kinetics. The changes in the absolute number of cells suggested two cell recruitment profiles: (i) a saliva‐dependent migration; and (ii) a migration dependent on the immune status of the host. These findings suggest that mosquito bites influence the skin microenvironment by inducing differential cell migration, which is dependent on the degree of host sensitization to salivary molecules. |
| Databáze: | OpenAIRE |
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