Genotypic Prediction of Human Immunodeficiency Virus Type 1 Tropism by Use of Plasma and Peripheral Blood Mononuclear Cells in the Routine Clinical Laboratory
| Autor: | Maria Geit, Jörg Berg, Christian Paar, Herbert Stekel |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Genotype Population HIV Infections Biology Peripheral blood mononuclear cell Virus Plasma Virology Blood plasma Humans education Tropism education.field_of_study virus diseases Sequence Analysis DNA biology.organism_classification Molecular Typing Viral Tropism DNA Viral Lentivirus Immunology HIV-1 Leukocytes Mononuclear Tissue tropism RNA Viral Viral load |
| Zdroj: | Journal of Clinical Microbiology. 49:2697-2699 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00336-11 |
| Popis: | We developed a sequencing assay for genotypic HIV-1 tropism determination. The assay allows examination of HIV RNA from plasma and HIV DNA from peripheral blood mononuclear cells (PBMC), including PBMC samples from patients with undetectable viral loads. Assessment of 100 pairs of plasma and PBMC samples showed a high concordance of 90%. With the limitations of population-based sequencing, the assay was found to be robust and suitable for the routine clinical laboratory. |
| Databáze: | OpenAIRE |
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