Prediction of Y haplogroup by polymerase chain reaction-reverse blot hybridization assay
| Autor: | Si-Keun Lim, Sehee Oh, Yang-Han Lee, Seoyong Kim, Kyungmyung Lee, Hyeyoung Lee, Jungho Kim, Sunyoung Park |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Forensic Genetics
Male 0106 biological sciences 0301 basic medicine Sequence analysis Human Y-chromosome DNA haplogroup Biology Y chromosome Polymerase Chain Reaction Polymorphism Single Nucleotide 01 natural sciences Biochemistry Haplogroup law.invention 03 medical and health sciences chemistry.chemical_compound Asian People law Genetics Humans SNP Molecular Biology Genotyping Polymerase chain reaction Chromosomes Human Y Reproducibility of Results 030104 developmental biology Haplotypes chemistry DNA 010606 plant biology & botany |
| Zdroj: | Genes & Genomics. 41:297-304 |
| ISSN: | 2092-9293 1976-9571 |
| DOI: | 10.1007/s13258-018-0761-6 |
| Popis: | The analysis of Y-SNPs from crime scene samples is helpful for investigators in narrowing down suspects by predicting biogeographical ancestry. In this study, a PCR-reverse blot hybridization assay (REBA) for predicting Y-chromosome haplogroups was employed to determine the major haplogroups worldwide, including AB, DE, C, C3, F, K, NO, O, O2, and O3 and evaluated. The REBA detects nine biallelic Y chromosome markers (M9, M89, M122, M145, M175, M214, M217, P31, and RPS4Y711) simultaneously using multiple probes. The REBA for Y-single nucleotide polymorphisms (SNP) genotyping was performed using 40 DNA samples from Asians—14 Koreans, 10 Indonesians, six Chineses, six Thais, and four Mongolians. 40 Asian samples were identified as haplogroup O2 (40%), O3 (32.5%), C3 (17.5%), O (7.5%) and K (2.5%). These cases were confirmed by DNA sequence analysis (κ = 1.00; P |
| Databáze: | OpenAIRE |
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