Top-down characterization of mouse core histones
| Autor: | Bingbing Xue, Zhixin Tian, Kaijie Xiao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Proteomics
Computational biology Top-down proteomics 01 natural sciences Methylation Histones Mice Animals Database search engine Databases Protein Spectroscopy Biological studies biology 010405 organic chemistry Chemistry 010401 analytical chemistry Protein species Acetylation 3T3 Cells 0104 chemical sciences Crosstalk (biology) Histone biology.protein Protein Processing Post-Translational |
| Zdroj: | Journal of mass spectrometry : JMS. 54(3) |
| ISSN: | 1096-9888 |
| Popis: | Histone post-translational modifications (PTMs) play various roles in chromatin-related cellular processes, and comprehensive analysis of these combinatorial PTMs at the intact protein level by top-down proteomics is the method of choice to reveal their crosstalk and biological functions. Here, we report our top-down characterization of the core histones from mouse fibroblasts cells NIH/3T3, which is a classic model used in many kinds of research. With nanoRPLC-MS/MS analysis and ProteinGoggle database search, 547 protein species were identified with spectrum-level FDR ≤ 1%, where PTMs in 51 protein species were unambiguously localized with PTM scores ≥1. High-resolution MS/MS data also allowed the unambiguous identification of acetylation instead of trimethylation. This study presents a general picture of combinatorial PTMs of mouse core histones, which serves as a basic reference for all future related biological studies. |
| Databáze: | OpenAIRE |
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