Transforming growth factor-beta 1 produced by vascular smooth muscle cells predicts fibrosis in the gastrocnemius of patients with peripheral artery disease
| Autor: | Holly DeSpiegelaere, George P Casale, Lauren C. Carpenter, Iraklis I. Pipinos, Mina Hanna, Panagiotis Koutakis, Zhen Zhu, Stanley A. Swanson, Duy M. Ha |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Vascular smooth muscle Myocytes Smooth Muscle Skeletal muscle 030204 cardiovascular system & hematology Muscle Smooth Vascular General Biochemistry Genetics and Molecular Biology Transforming Growth Factor beta1 Masson's trichrome stain Extracellular matrix Peripheral Arterial Disease 03 medical and health sciences 0302 clinical medicine Microvasculature Fibrosis Internal medicine Vascular smooth muscle cells medicine Humans Myocyte Muscle Skeletal Fibroblast Myopathy Demography Medicine(all) Peripheral artery disease Biochemistry Genetics and Molecular Biology(all) business.industry Research General Medicine Fibroblasts Middle Aged medicine.disease 3. Good health 030104 developmental biology medicine.anatomical_structure Endocrinology Transforming growth factor-beta 1 Case-Control Studies Microvessels Female Collagen medicine.symptom business |
| Zdroj: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| DOI: | 10.1186/s12967-016-0790-3 |
| Popis: | Background Lower leg ischemia, myopathy, and limb dysfunction are distinguishing features of peripheral artery disease (PAD). The myopathy of PAD is characterized by myofiber degeneration in association with extracellular matrix expansion, and increased expression of transforming growth factor-beta 1 (TGF-β1; a pro-fibrotic cytokine). In this study, we evaluated cellular expression of TGF-β1 in gastrocnemius of control (CTRL) and PAD patients and its relationship to deposited collagen, fibroblast accumulation and limb hemodynamics. Methods Gastrocnemius biopsies were collected from PAD patients with claudication (PAD-II; N = 25) and tissue loss (PAD-IV; N = 20) and from CTRL patients (N = 20). TGF-β1 in slide-mounted specimens was labeled with fluorescent antibodies and analyzed by quantitative wide-field, fluorescence microscopy. We evaluated co-localization of TGF-β1 with vascular smooth muscle cells (SMC) (high molecular weight caldesmon), fibroblasts (TE-7 antigen), macrophages (CD163), T cells (CD3) and endothelial cells (CD31). Collagen was stained with Masson Trichrome and collagen density was determined by quantitative bright-field microscopy with multi-spectral imaging. Results Collagen density increased from CTRL to PAD-II to PAD-IV specimens (all differences p |
| Databáze: | OpenAIRE |
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