3D printed biofunctionalized scaffolds for microfracture repair of cartilage defects
| Autor: | Ting Guo, Max J. Lerman, Jonathan D. Packer, Maeesha Noshin, Hannah B. Baker, Yu Chen, John P. Fisher, Evin Taskoy, Julia P. Ringel, Qinggong Tang, Sean J. Meredith |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cartilage Articular Scaffold Fractures Stress Biophysics Type II collagen Bioengineering 02 engineering and technology Article Cell Line Biomaterials 03 medical and health sciences Chondrocytes medicine Cell Adhesion Animals Humans Aggrecans Aggrecan Tissue Scaffolds Chemistry Cartilage Regeneration (biology) Endogenous regeneration Bioprinting 021001 nanoscience & nanotechnology Chondrogenesis 030104 developmental biology medicine.anatomical_structure Immobilized Proteins Mechanics of Materials Printing Three-Dimensional Ceramics and Composites Female Bone marrow Rabbits 0210 nano-technology Biomedical engineering |
| Zdroj: | Biomaterials. 185 |
| ISSN: | 1878-5905 |
| Popis: | While articular cartilage defects affect millions of people worldwide from adolescents to adults, the repair of articular cartilage defects still remains challenging due to the limited endogenous regeneration of the tissue and poor integration with implants. In this study, we developed a 3D-printed scaffold functionalized with aggrecan that supports the cellular fraction of bone marrow released from microfracture, a widely used clinical procedure, and demonstrated tremendous improvement of regenerated cartilage tissue quality and joint function in a lapine model. Optical coherence tomography (OCT) revealed doubled thickness of the regenerated cartilage tissue in the group treated with our aggrecan functionalized scaffold compared to standard microfracture treatment. H&E staining showed 366 ± 95 chondrocytes present in the unit area of cartilage layer with the support of bioactive scaffold, while conventional microfracture group showed only 112 ± 26 chondrocytes. The expression of type II collagen appeared almost 10 times higher with our approach compared to normal microfracture, indicating the potential to overcome the fibro-cartilage formation associated with the current microfracture approach. The therapeutic effect was also evaluated at joint function level. The mobility was evaluated using a modified Basso, Beattie and Bresnahan (BBB) scale. While the defect control group showed no movement improvement over the course of study, all experimental groups showed a trend of increasing scores over time. The present work developed an effective method to regenerate critical articular defects by combining a 3D-printed therapeutic scaffold with the microfracture surgical procedure. This biofunctionalized acellular scaffold has great potential to be applied as a supplement for traditional microfracture to improve the quality of cartilage regeneration in a cost and labor effective way. |
| Databáze: | OpenAIRE |
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