Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers. II. Loading of the Hemoderivative Platelet Lysate
| Autor: | Marika Tenci, Claudia Del Fante, Giuseppina Sandri, Silvia Rossi, Giovanni Nicoletti, Franca Ferrari, Carla Caramella, Michela Mori, Federica Riva, Maria Cristina Bonferoni |
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| Rok vydání: | 2016 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Chemistry Pharmaceutical Glycine Glutamic Acid Pharmaceutical Science Human skin 02 engineering and technology Absorption (skin) Administration Cutaneous 030226 pharmacology & pharmacy Sericin Chitosan 03 medical and health sciences chemistry.chemical_compound Freeze-drying 0302 clinical medicine Skin Ulcer medicine Animals Humans Sericins Fibroblast Cells Cultured Cell Proliferation Platelet-Derived Growth Factor Wound Healing Chromatography integumentary system Fibroblasts 021001 nanoscience & nanotechnology Bandages Porifera Surgery Solutions Freeze Drying medicine.anatomical_structure chemistry Platelet lysate 0210 nano-technology Wound healing |
| Zdroj: | Journal of Pharmaceutical Sciences. 105:1188-1195 |
| ISSN: | 0022-3549 |
| Popis: | Platelet lysate (PL) was loaded into dressings based on chitosan glutamate (CSG) low and high molecular weight, sericin (Ser), and glycine (Gly). A synergic effect of Ser and PL on fibroblast proliferation was proved in vitro. Two different PL loading approaches were considered: the first provided to prepare dressings by freeze-drying a mixture of PL and CSG/Gly/Ser solution, the second approach consisted in the extemporarily loading of PL in the CSG/Gly/Ser freeze-dried dressings. As for the first approach, PL loading did not produce any variation in dressing mechanical properties. Such dressings absorbed a high amount (about 8-fold of dry weight) of phosphate-buffered saline (fluid mimicking wound exudate), forming a gel with pseudoplastic and elastic properties. Platelet-derived growth factor AB assay indicated that neither freeze-drying nor the excipients alter PL growth factor content. As for the second approach, mechanical and rheological properties of the gel formed upon PL absorption enabled to choose a PL loading of about 90 μL/cm(2). Upon contact with fibroblasts, all PL loaded formulations increased the number not only of viable cells but also of those in the proliferative phase. Histological studies effected on human skin strips pointed out the positive effect of PL loaded dressings on dermal matrix reconstruction. |
| Databáze: | OpenAIRE |
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