| Autor: |
Andrei V. Filatov, Andrei V. Perepelov, Alexander S. Shashkov, Gennady L. Burygin, Natalia E. Gogoleva, Yuriy A. Khlopko, Vyacheslav S. Grinev |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Carbohydrate research. 508 |
| ISSN: |
1873-426X |
| Popis: |
The O-antigen (O-polysaccharide) is an essential component of lipopolysaccharide on the surface of Gram-negative bacteria and plays an important role in interaction with host organisms. In this study, we investigated the chemical structure and characterized the gene cluster of Enterobacter cloacae K7 O-antigen. As judged by sugar analyses along with NMR spectroscopy data, E. cloacae K7 antigen has a tetrasaccharide O-unit with the following structure: →8)-β-Psep5Ac7Ac-(2 → 2)-β-l-Rhap-(1 → 4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1→ The O-antigen gene cluster of E. cloacae K7 between conserved genes galF and gnd was sequenced. Most genes necessary for the O-antigen synthesis were found in the cluster and their functions were tentatively assigned by comparison with sequences in the available databases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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