An enzyme immunoassay for the screening of monoclonal antibodies to cyclosporin
| Autor: | M.H.V. Van Regenmortel, Valérie F. J. Quesniaux, K. Himmelspach |
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| Rok vydání: | 1985 |
| Předmět: |
Ratón
medicine.drug_class medicine.medical_treatment Immunology Cyclosporin metabolites Cyclosporins Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Biology Monoclonal antibody Nephrotoxicity Immunoenzyme Techniques Mice polycyclic compounds medicine Animals Immunology and Allergy chemistry.chemical_classification Hybridomas medicine.diagnostic_test Antibodies Monoclonal Immunosuppression Virology Enzyme chemistry Immunization Immunoassay |
| Zdroj: | Immunology Letters. 9:99-104 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/0165-2478(85)90018-5 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) has been developed which allows hybridoma cell cultures to be tested for the presence of monoclonal antibodies specific for cyclosporin A. Immunization of mice wit free cyclosporin was found to be preferable to immunization with cyclosoporin-carrier conjugates. It is hoped that the availability of monoclonal antibodies to cyclosporin will clarify the contribution of cyclosporin metabolites to immunosuppression and nephrotoxicity. |
| Databáze: | OpenAIRE |
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