Phototriggered Secretion of Membrane Compartmentalized Bioactive Agents
| Autor: | Zachary L. Rodgers, Song Ding, Weston J. Smith, Robert M. Hughes, Nathan P. Oien, David S. Lawrence, Christina M. Marvin |
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| Rok vydání: | 2016 |
| Předmět: |
Boron Compounds
Erythrocytes Fluorophore genetic structures Paclitaxel 010402 general chemistry 01 natural sciences Article Catalysis chemistry.chemical_compound Humans Secretion Molecular Structure 010405 organic chemistry Chemistry Biological activity General Chemistry Photochemical Processes 0104 chemical sciences Vitamin B 12 Methotrexate Membrane Biochemistry Covalent bond Biophysics sense organs BODIPY Colchicine Linker HeLa Cells Conjugate |
| Zdroj: | Angewandte Chemie International Edition. 55:16080-16083 |
| ISSN: | 1433-7851 |
| Popis: | A strategy for the light-activated release of bioactive compounds (BODIPY, colchicine, paclitaxel, and methotrexate) from membrane-enclosed depots is described. We have found that membrane-permeable bioagents can be rendered membrane impermeable by covalent attachment to cobalamin (Cbl) through a photocleavable linker. These Cbl-bioagent conjugates are imprisoned within lipid-enclosed compartments in the dark, as exemplified by their retention in the interior of erythrocytes. Subsequent illumination drives the secretion of the bioactive species from red blood cells. Photorelease is triggered by wavelengths in the red, far-red, and near-IR regions, which can be pre-assigned by affixing a fluorophore with the desired excitation wavelength to the Cbl-bioagent conjugate. Pre-assigned wavelengths allow different biologically active compounds to be specifically and unambiguously photoreleased from common carriers. |
| Databáze: | OpenAIRE |
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