Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica

Autor: Chun-Xiang You, Peng-Fei Zheng, Xun Wang, Yu-Ying Yang, Zhen-Lu Zhang, Yu-Jin Hao
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Light
Arabidopsis
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
Gene Expression Regulation
Plant
Gene expression
Basic Helix-Loop-Helix Transcription Factors
Arabidopsis thaliana
lcsh:QH301-705.5
Abscisic acid
Spectroscopy
Phylogeny
Plant Proteins
Genetics
biology
gene expression analysis
food and beverages
phytochrome-interacting factor
General Medicine
Computer Science Applications
Malus
Multigene Family
Plant hormone
Phytochrome
transcription activation activity
Plant Development
protein localization
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Stress
Physiological
Physical and Theoretical Chemistry
Molecular Biology
Transcription factor
Gene
Abiotic stress
Arabidopsis Proteins
Organic Chemistry
fungi
ABA sensitivity
biology.organism_classification
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
010606 plant biology & botany
Abscisic Acid
Transcription Factors
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 19
International Journal of Molecular Sciences, Vol 21, Iss 7350, p 7350 (2020)
ISSN: 1422-0067
Popis: Phytochrome-interacting factors (PIFs), members of the basic helix-loop-helix transcription factor family that have been extensively investigated in Arabidopsis thaliana, play essential roles in plant growth and development. However, PIF members have not been systematically investigated in apples, a worldwide perennial woody crop of economic importance. Here, seven PIF genes were identified from the Malus ×
domestica reference genome. Chromosomal locations, gene structures, and phylogenetic relationships of these members were analyzed. Analysis of cis-acting elements in promoter regions of MdPIF genes indicated that various elements were related to light, abiotic stress, and plant hormone responsiveness. Subsequently, subcellular localization and transcriptional activity analysis revealed that MdPIFs were typical nuclear transcription factors with transcriptional activation ability. Expression analysis demonstrated that MdPIF genes had different gene expression patterns for various abiotic factors. Moreover, overexpressed MdPIF4 reduced the sensitivity of apple calluses to abscisic acid (ABA). Our work lays foundations for further investigation of PIF functions in plant growth and development in apples.
Databáze: OpenAIRE
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