Role of the Sec22b/E-Syt complex in neurite growth and ramification
| Autor: | Lydia Danglot, Thomas Binz, Alessandra Gallo, Christian Vannier, Francesca Giordano, Bailey Hewlett, Thierry Galli |
|---|---|
| Přispěvatelé: | Institut de psychiatrie et neurosciences de Paris (IPNP - U1266 Inserm), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Ecole des Neurosciences de Paris Île de France (ENP), Ecole des Neurosciences de Paris, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Trafic lipidique et sites de contact membranaire (COAST), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Medizinische Hochschule Hannover (MHH), GHU Paris Psychiatrie et Neurosciences, ANR-16-CE16-0012,MeTDePaDi,Défauts de Traffic Membranaire dans la Maladie de Parkinson(2016), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), SERRE, Marie-Claude |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Membrane contact site
Neurite [SDV]Life Sciences [q-bio] Mutant Membrane trafficking Biology Endoplasmic Reticulum Synaptotagmin 1 Axonal growth 03 medical and health sciences Synaptotagmins 0302 clinical medicine Neurites Humans 030304 developmental biology Filopodia 0303 health sciences Endoplasmic reticulum Cell Membrane Cell Biology Cell biology [SDV] Life Sciences [q-bio] Transmembrane domain SNARE Lipid-transfer protein SNARE Proteins Plant lipid transfer proteins 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cell Science Journal of Cell Science, 2020, ⟨10.1242/jcs.247148⟩ Journal of Cell Science, Company of Biologists, 2020, ⟨10.1242/jcs.247148⟩ |
| ISSN: | 0021-9533 1477-9137 |
| DOI: | 10.1242/jcs.247148⟩ |
| Popis: | Axons and dendrites are long and often ramified neurites that need particularly intense plasma membrane (PM) expansion during the development of the nervous system. Neurite growth depends on non-fusogenic Sec22b–Stx1 SNARE complexes at endoplasmic reticulum (ER)–PM contacts. Here, we show that Sec22b interacts with members of the extended synaptotagmin (E-Syt) family of ER lipid transfer proteins (LTPs), and this interaction depends on the longin domain of Sec22b. Overexpression of E-Syts stabilizes Sec22b–Stx1 association, whereas silencing of E-Syts has the opposite effect. Overexpression of wild-type E-Syt2, but not mutants unable to transfer lipids or attach to the ER, increase the formation of axonal filopodia and ramification of neurites in developing neurons. This effect is inhibited by a clostridial neurotoxin cleaving Stx1, and expression of the Sec22b longin domain and a Sec22b mutant with an extended linker between the SNARE and transmembrane domains. We conclude that Sec22b–Stx1 ER–PM contact sites contribute to PM expansion by interacting with LTPs, such as E-Syts. This article has an associated First Person interview with the first author of the paper. |
| Databáze: | OpenAIRE |
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