p-21-Activated kinase-1, -4 and -6 and estrogen receptor expression pattern in normal placenta and gestational trophoblastic diseases
| Autor: | Melina Shoni, Cynthia A. Jimenez, Allison F. Vitonis, Ross S. Berkowitz, Shu-Wing Ng, Bradley J. Quade, Zoltan Nagymanyoki |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Placenta Estrogen receptor Biology Andrology Pathogenesis Syncytiotrophoblast Downregulation and upregulation Pregnancy Internal medicine Mole medicine Humans Choriocarcinoma Gestational Trophoblastic Disease reproductive and urinary physiology Cytotrophoblast Kinase Intermediate trophoblast Obstetrics and Gynecology medicine.disease Immunohistochemistry Isoenzymes Endocrinology medicine.anatomical_structure Receptors Estrogen p21-Activated Kinases Oncology Uterine Neoplasms embryonic structures Female |
| Zdroj: | Gynecologic Oncology. 131:759-763 |
| ISSN: | 0090-8258 |
| DOI: | 10.1016/j.ygyno.2013.09.010 |
| Popis: | Objectives To delineate the potential role of p21-activated kinases (PAKs) in the pathogenesis of gestational trophoblastic diseases (GTD) by defining the expression pattern of PAK-1, -4 and -6 and their potential implication in estrogen receptor (ER) regulation of normal placental tissue and GTD. Methods We evaluated immunohistochemically 10 normal first-trimester placentas (NP), 10 partial moles (PM), 15 complete moles (CM) and 3 choriocarcinomas (CCA) for PAK-1, PAK-4, PAK-6 and ER expression intensity and localization. Staining outcomes were assessed utilizing non-parametric Kruskal–Wallis one-way analysis of variance test followed by pairwise Wilcoxon Rank Sum tests. Statistical significance was determined by two-sided p -value of Results In NP, PAK-6 immunoreactivity was predominantly cytoplasmic. Compared to NP, PM and CM demonstrated significant increase of cytoplasmic PAK-6 in cytotrophoblast ( p =0.012, p =0.033 respectively), accompanied by significantly increased nuclear immunoreactivity in cytotrophoblast ( p =0.008, p =0.045 respectively) and intermediate trophoblast ( p =0.003, p =0.015 respectively). PAK-4 was found significantly upregulated in both cytoplasmic and nuclear compartments of cytotrophoblast and syncytiotrophoblast in PM ( p =0.004 and p =0.002 for cytotrophoblast; p =0.018 and p =0.002 for syncytiotrophoblast, respectively) and CM ( p =0.001 and p =0.001 for cytotrophoblast; p =0.002 and p =0.001 for syncytiotrophoblast, respectively) when compared to NP, whereas PAK-1 expression was significantly reduced in the syncytiotrophoblast of PM ( p =0.025 for cytoplasm and p =0.008 for nucleus). Nuclear expression of ER was undetectable in all stained samples. Conclusion Our results reveal PAK-6 upregulation in GTD compared to NP. The absence of nuclear expression of ER might stem in part from the repressive effect of PAK-6 in trophoblastic tissue. |
| Databáze: | OpenAIRE |
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