Pyrogen testing of lipid-based TPN using Mono Mac 6 monocyte cell line and DELFIA
| Autor: | J D Christensen, E W Hansen, L Moesby |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
Quality Control Lipopolysaccharide medicine.medical_treatment Fluoroimmunoassay Cross Reactions Immunofluorescence Monocytes Cell Line chemistry.chemical_compound Europium medicine Humans Pharmacology (medical) Pharmacology medicine.diagnostic_test Chemistry Interleukin-6 Pyrogens Monocyte Molecular biology In vitro Cytokine medicine.anatomical_structure Biochemistry Cell culture Immunoassay Tumor necrosis factor alpha Biological Assay Indicators and Reagents Parenteral Nutrition Total Drug Contamination |
| Zdroj: | Journal of clinical pharmacy and therapeutics. 22(5-6) |
| ISSN: | 0269-4727 |
| Popis: | SUMMARY Objective: Measurement of lipopolysaccharide (LPS) induced interleukin-6 (IL-6) secretion in Mono Mac 6 cells. Method: Dissociation-enhanced lanthanide fluoro immunoassay (DELFIA), a technique based on time-resolved fluorescence. Results: A comparison of DELFIA and the B9 bio-assay showed a correlation between the results of the two assays. DELFIA did not show any cross-reactivity with the human cytokines TNFα, IL-1β, IL-1α, IL-2, IL-4, IFNγ, murine IL-6 or LPS. In aqueous solution the detection limit was 2.5 pg LPS/ml, and in a 25% dilution of a lipid-based total parenteral nutrition (TPN) product it was 25 pg LPS/ml. TPN did not interfere with the DELFIA assay, but the IL-6 secretion from the Mono Mac 6 cells was inhibited at TPN concentrations above 25%. Conclusion: A combination of Mono Mac 6 cells with DELFIA was found to be a reliable method for detecting LPS, but further validation by independent laboratories is justified. |
| Databáze: | OpenAIRE |
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