A functional extracellular matrix biomaterial derived from ovine forestomach
| Autor: | Evan Floden, Madhusudan Vasudevamurthy, Rene J. McLaughlin, Neil J. Fisher, Brian Roderick Ward, Sandi G. Dempsey, Barnaby C. H. May, Sharleen M. Irvine, Stan Lun, Leonardo Negron, Keryn Dallas Johnson |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Biophysics Bioengineering Biocompatible Materials PC12 Cells Microbiology Biomaterials Extracellular matrix Laminin medicine Cell Adhesion Animals Humans Protein Isoforms Regeneration Keratinocyte migration Fibroblast Basement membrane Sheep biology Stomach Biomaterial Cell Differentiation Cell biology Extracellular Matrix Rats Fibronectin medicine.anatomical_structure Mechanics of Materials Ceramics and Composites biology.protein Fibroblast Growth Factor 2 Collagen Wound healing |
| Zdroj: | Biomaterials. 31(16) |
| ISSN: | 1878-5905 |
| Popis: | Extracellular matrix (ECM) based biomaterials have an established place as medical devices for wound healing and tissue regeneration. In the search for biomaterials we have identified ovine forestomach matrix (OFM), a thick, large format ECM which is biochemically diverse and biologically functional. OFM was purified using an osmotic process that was shown to reduce the cellularity of the ECM and aid tissue delamination. OFM produced using this technique was shown to retain residual basement membrane components, as evidence by the presence of laminin and collagen IV. The collagenous microarchitecture of OFM retained many components of native ECM including fibronectin, glycosaminoglycans, elastin and fibroblast growth factor basic. OFM was non-toxic to mammalian cells and supported fibroblast and keratinocyte migration, differentiation and infiltration. OFM is a culturally acceptable alternative to current collagen-based biomaterials and has immediate clinical applications in wound healing and tissue regeneration. |
| Databáze: | OpenAIRE |
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