Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury
| Autor: | Patrícia Amado, Yara Marcondes Machado Castiglia, Renata Pinheiro Módolo, Marilia P. Modolo, André Roberto Bussmann, Maria Aparecida Custódio Domingues, Norma Sueli Pinheiro Módolo, Marcos Antonio Marton Filho |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), ABC Medical School |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Pathology RD1-811 Antimetabolites medicine.medical_treatment Allopurinol Urology Ischemia Renal function Kidney Biomarkers Pharmacological Random Allocation chemistry.chemical_compound Lipocalin-2 Proto-Oncogene Proteins Laparotomy medicine Animals Rats Wistar Creatinine business.industry Acute kidney injury Interleukin-18 Histology Acute Kidney Injury medicine.disease Lipocalins Rats chemistry Reperfusion Injury Surgery Interleukin 18 business Biomarkers Acute-Phase Proteins medicine.drug |
| Zdroj: | Acta Cirúrgica Brasileira v.29 n.8 2014 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 29, Issue: 8, Pages: 515-521, Published: AUG 2014 SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Acta Cirurgica Brasileira, Vol 29, Iss 8, Pp 515-521 (2014) |
| ISSN: | 0102-8650 |
| Popis: | Made available in DSpace on 2015-02-02T12:39:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-08-01Bitstream added on 2015-02-02T13:07:52Z : No. of bitstreams: 1 S0102-86502014000800515.pdf: 315724 bytes, checksum: 5e429d826b2eebfd4baa578c154d2e90 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) PURPOSE: To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM 1 and IL 18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury. METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg·kg 1·d 1; and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg·kg 1·d 1. The NGALp, NGALu, KIM 1, IL 18 and creatinine levels and the kidney histology were analysed. The significance level was established as p S ≈ CA. The NGALp, NGALu and IL 18 levels exhibited similar behaviour in all the groups. KIM 1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA. CONCLUSION: Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury. Sao Paulo State University Botucatu Medical School UNESP Botucatu Medical School ABC Medical School UNESP Botucatu Medical School Department of Pathology UNESP Botucatu Medical School Department of Anaesthesiology Sao Paulo State University Botucatu Medical School UNESP Botucatu Medical School UNESP Botucatu Medical School Department of Pathology UNESP Botucatu Medical School Department of Anaesthesiology |
| Databáze: | OpenAIRE |
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