LuxR Solos in the Plant Endophyte Kosakonia sp. Strain KO348
| Autor: | Alexander Martin Geller, Iris Bertani, Susan Mosquito, Xianfa Meng, Sonia Covaceuszach, Michael P. Myers, Cristina Bez, Giulia Devescovi, Vittorio Venturi, Asaf Levy |
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| Rok vydání: | 2020 |
| Předmět: |
Subfamily
education Homoserine Applied Microbiology and Biotechnology Endophyte 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Enterobacteriaceae Environmental Microbiology Endophytes Amino Acid Sequence bacteria Symbiosis Phylogeny LuxR solos 030304 developmental biology Genetics 0303 health sciences Ecology biology 030306 microbiology Host (biology) food and beverages Oryza biochemical phenomena metabolism and nutrition biology.organism_classification Repressor Proteins Quorum sensing chemistry Trans-Activators Proteobacteria cell-cell signaling endophyte gene regulation Cell-cell signaling Sequence Alignment Bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and environmental microbiology 86 (2020): e00622-20–e00622-20. doi:10.1128/AEM.00622-20 info:cnr-pdr/source/autori:Mosquito, Susan; Meng, Xianfa; Devescovi, Giulia; Bertani, Iris; Geller, Alexander M.; Levy, Asaf; Myers, Michael P.; Bez, Cristina; Covaceuszach, Sonia; Venturi, Vittorio/titolo:LuxR Solos in the Plant Endophyte Kosakonia sp. Strain KO348/doi:10.1128%2FAEM.00622-20/rivista:Applied and environmental microbiology (Print)/anno:2020/pagina_da:e00622-20/pagina_a:e00622-20/intervallo_pagine:e00622-20–e00622-20/volume:86 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00622-20 |
| Popis: | Cell-cell signaling in bacteria allows a synchronized and coordinated behavior of a microbial community. LuxR solos represent a subfamily of proteins in proteobacteria which most commonly detect and respond to signals produced exogenously by other microbes or eukaryotic hosts. Here, we report that a plant-beneficial bacterial endophyte belonging to the novel genus of Kosakonia possesses two LuxR solos; one is involved in the detection of exogenous N-acyl homoserine lactone quorum sensing signals and the other in detecting a compound(s) produced by the host plant. These two Kosakonia LuxR solos are therefore most likely involved in interspecies and interkingdom signaling. Endophytes are microorganisms that live inside plants and are often beneficial for the host. Kosakonia is a novel bacterial genus that includes several species that are diazotrophic and plant associated. This study revealed two quorum sensing-related LuxR solos, designated LoxR and PsrR, in the plant endophyte Kosakonia sp. strain KO348. LoxR modeling and biochemical studies demonstrated that LoxR binds N-acyl homoserine lactones (AHLs) in a promiscuous way. PsrR, on the other hand, belongs to the subfamily of plant-associated-bacterium (PAB) LuxR solos that respond to plant compounds. Target promoter studies as well as modeling and phylogenetic comparisons suggest that PAB LuxR solos are likely to respond to different plant compounds. Finally, LoxR is involved in the regulation of T6SS and PsrR plays a role in root endosphere colonization. IMPORTANCE Cell-cell signaling in bacteria allows a synchronized and coordinated behavior of a microbial community. LuxR solos represent a subfamily of proteins in proteobacteria which most commonly detect and respond to signals produced exogenously by other microbes or eukaryotic hosts. Here, we report that a plant-beneficial bacterial endophyte belonging to the novel genus of Kosakonia possesses two LuxR solos; one is involved in the detection of exogenous N-acyl homoserine lactone quorum sensing signals and the other in detecting a compound(s) produced by the host plant. These two Kosakonia LuxR solos are therefore most likely involved in interspecies and interkingdom signaling. |
| Databáze: | OpenAIRE |
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