Evaluation of crystal violet decolorization assay for minimal inhibitory concentration detection of primary antituberculosis drugs against Mycobacterium tuberculosis isolates

Autor: Asuman Birinci, Ahmet Yilmaz Coban, Yeliz Tanrıverdi Çaycı, Belma Durupinar, Meltem Uzun, Ahmet Ugur Akbal
Přispěvatelé: OMÜ
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
030106 microbiology
030231 tropical medicine
lcsh:QR1-502
Antitubercular Agents
Microbial Sensitivity Tests
multi drug resistance
Biology
lcsh:Microbiology
Microbiology
Mycobacterium tuberculosis
03 medical and health sciences
chemistry.chemical_compound
Minimum inhibitory concentration
0302 clinical medicine
Drug Resistance
Multiple
Bacterial
Tuberculosis
Multidrug-Resistant
medicine
Isoniazid
Humans
Crystal violet
antituberculosis drugs
Ethambutol
Articles
susceptibility testing
biology.organism_classification
bacterial infections and mycoses
crystal violet decolorization assay
Multiple drug resistance
chemistry
Streptomycin
Biological Assay
Gentian Violet
Indicators and Reagents
Rifampin
Rifampicin
medicine.drug
Zdroj: Memórias do Instituto Oswaldo Cruz
Memórias do Instituto Oswaldo Cruz., Iss 0 (2016)
Memórias do Instituto Oswaldo Cruz, Volume: 111, Issue: 7, Pages: 454-459, Published: 10 JUN 2016
Popis: Akbal, Ahmet Ugur/0000-0002-0627-5326 WOS: 000380112500006 PubMed: 27304025 In this study we evaluated the crystal violet decolorization assay (CVDA) for detection of minimum inhibitory concentration (MIC) of antituberculosis drugs. 53 isolates were tested in this study and 13 of them were multidrug resistant (MDR) isolates. The antibiotics concentrations were 2-0.06 mg/L for isoniazid (INH) and rifampicin (RIF) and were 16-0.25 mg/L for streptomycin (STM) and ethambutol (EMB). Crystal violet (CV-25 mg/L) was added into the microwells on the seventh day of incubation and incubation was continued until decolorization. Decolorization of CV was the predictor of bacterial growth. Overall agreements for four drugs were detected as 98.1%, and the average time was detected as 9.5 +/- 0.89 day after inoculation. One isolate for INH and two isolates for STM were determined resistant in the reference method, but susceptible by the CVDA. One isolate was susceptible to EMB by the reference method, but resistant by the CVDA. All results were concordant for RIF. This study shows that CVDA is a rapid, reliable and suitable for determination of MIC values of Mycobacterium tuberculosis. And it can be used easily especially in countries with limited-sources.
Databáze: OpenAIRE
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