Contrasting effects of alpha- and beta-androstenediol on oncogenic myeloid cell lines in vitro
| Autor: | P N Huynh, R M Loria |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Androstenediol
Programmed cell death Cell Survival Immunology Alpha (ethology) Apoptosis HL-60 Cells DNA Fragmentation Biology Cell Line chemistry.chemical_compound Mice Structure-Activity Relationship Cyclic AMP Immunology and Allergy Animals Humans Beta (finance) Macrophages Stereoisomerism Cell Biology Molecular biology Growth Inhibitors chemistry Cell culture DNA fragmentation Trypan blue |
| Zdroj: | Journal of leukocyte biology. 62(2) |
| ISSN: | 0741-5400 |
| Popis: | The in vitro effects of 17αAED, the isomer of 5-androstene-3β,17β diol (17βAED) on the basal growth of murine RAW 264.7, P388D1, and human HL-60 cells were investigated. 17αAED treatment of RAW cells for 48 h reduced total cell number without increasing cell death as detected by trypan blue exclusion. At these doses, DNA synthesis as measured by [3H]thymidine incorporation was suppressed by as much as 65%, P < 0.05. This effect was time- and dose-dependent and reversible on removal of the steroid. Similar results were obtained with P388D1 and human HL-60 cell lines. At 50 nM or above, 17αAED induced apoptosis in RAW cells and HL-60 as detected by transmission electron microscopy and TUNEL assays. By contrast, treating cells with the isomer 17βAED had no such effect. These data suggest that the balance between the anti-proliferative effect of 17αAED and the proliferative effects of 17βAED may determine the overall level of myelopoiesis. |
| Databáze: | OpenAIRE |
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