In vitro ability of Staphylococcus aureus isolates from bacteraemic patients with and without metastatic complications to invade vascular endothelial cells
| Autor: | Kang Won Choe, Myoung Don Oh, Kyoung Wha Park, Sung-Han Kim, Wan Beom Park, Hong Bin Kim, Jae Hyun Cho, Nam Joong Kim, Ji Whan Bang, Cheol-In Kang, Yeong Seon Lee |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Adult Male Cytoplasm Staphylococcus aureus Micrococcaceae Colony Count Microbial Bacteremia medicine.disease_cause Microbiology Metastasis Interquartile range medicine Cytotoxic T cell Humans Aged biology Endothelial Cells General Medicine Middle Aged Staphylococcal Infections biology.organism_classification medicine.disease In vitro Endothelial stem cell Community-Acquired Infections Immunology Female |
| Zdroj: | Journal of medical microbiology. 56(Pt 10) |
| ISSN: | 0022-2615 |
| Popis: | Invasion of vascular endothelial cells is thought to be a critical step in the development of metastatic infections in patients withStaphylococcus aureusbacteraemia. This study was designed to evaluate the association between the ability to invade endothelial cells and metastatic infection byS. aureus. Patients with metastatic infection were identified among those with community-acquiredS. aureusbacteraemia in a tertiary referral hospital. Patients with simple bacteraemia caused byS. aureusover the same period served as the control group. The ability of each clinical isolate to invade endothelial cells was evaluated by counting the number of intracellular organisms 1 h after inoculation onto human umbilical vein endothelial cellsin vitro. The cytotoxic activity of intracellularS. aureuswas determined 24 h after internalization, and expressed as the percentage of cells killed. The clinical isolates varied in invasiveness and cytotoxicity. The median invasiveness, relative toS. aureusreference strain ATCC 29213, was 145 % in the cases (n=10) [interquartile range (IQR) 103–160] and 153 % (IQR 111–173) in the controls (n=11;P=0.44). The median cytotoxicity was 59.4 % (IQR 47–68) in the cases and 65.2 % (IQR 50–74) in the controls (P=0.44). Differences in the ability ofS. aureusto invade and destroy vascular endothelial cellsin vitrowere not associated with the development of metastatic complications in patients withS. aureusbacteraemia. This implies that the invasiveness and toxicity ofS. aureusfor endothelial cells may not be major determinants of metastatic infection. |
| Databáze: | OpenAIRE |
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