The treatment of prednisone in mild diabetic rats: Biochemical parameters and cell response
| Autor: | Mariana Pirani Rocha Machado, Amanda Lima Deluque, Gustavo Tadeu Volpato, Kleber Eduardo de Campos, Aline Zanatta Schavinski |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Federal University of Mato Grosso (UFMT), Universidade Federal de Mato Grosso do Sul (UFMS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Blood Glucose medicine.medical_specialty Food intake Endocrinology Diabetes and Metabolism 030204 cardiovascular system & hematology Immunoglobulin G Diabetes Mellitus Experimental 03 medical and health sciences Random Allocation 0302 clinical medicine Insulin resistance Lipometabolism Prednisone Internal medicine Diabetes mellitus Glucose Intolerance medicine Immunology and Allergy Animals Glucocorticoids Immunosuppressant biology business.industry Insulin tolerance test Body Weight Diabetes Glucose Tolerance Test medicine.disease Rats Adult life 030104 developmental biology Endocrinology Treatment Outcome biology.protein Cell response Glycometabolism Insulin Resistance business medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Web of Science |
| Popis: | Made available in DSpace on 2020-12-10T20:01:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Fundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT), Cuiaba, Brazil (2014-2016) Background: Limited studies have been canied out with prednisone (PRED) in treatment by glucose intolerant individuals, even in this model the animals presented low blood glucose levels at adulthood, by the high regenerative capacity of beta-cell. Objective: The aim was to evaluate the effects of the treatment of PRED in mild diabetes on biochemical and immunological biomarkers. Methods: Rats were randomly divided into four groups: control (C), treated control C+PRED (treatment of 1.25 mg/Kg/day PRED); diabetic DM (mild diabetes) and treated diabetic DM PRED (treatment with same dose as C+PRED group). Untreated groups received vehicle, adjusted volume to body weight. The treatment lasted 21 days and measured body weight, food and water intake, and glycemia weekly. In the 3rd week, the Oral Glucose Tolerance Test (OGTT) and the Insulin Tolerance Test (ITT) was performed. On the last day, the rats were killed and the blood was collected for biochemical analyzes, leukogram and imm.unoglobulin G levels. Results: There was a significant decrease in body weight in mild diabetes; however, the treatment in diabetic groups increased food intake, glycemia, and the number of total leukocytes, lymphocytes and neutrophils. On the other hand, it decreased the levels of triglycerides, high -density and very low density lipoproteins. In addition, diabetic groups showed glucose intolerance and mild insulin resistance, confirming that this model induces glucose intolerant in adult life. Conclusion: The results showed that the use of prednisone is not recommended for glucose intolerant individuals and should he replaced in order to not to aggravate this condition. Sao Paulo State Univ UNESP, Inst Biosci, Postgrad Program Pharmacol & Biotechnol, Botucatu, SP, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Physiol, Sao Paulo, Brazil Fed Univ Mato Grosso UFMT, Inst Biol Sci & Hlth, Lab Syst Physiol & Reprod Toxicol, Barra Do Garcas, Mato Grosso, Brazil Sao Paulo State Univ UNESP, Inst Biosci, Postgrad Program Pharmacol & Biotechnol, Botucatu, SP, Brazil Fundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT), Cuiaba, Brazil (2014-2016): 002 - 004/2015 Fundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT), Cuiaba, Brazil (2014-2016): 36016.541.21414.17082016 |
| Databáze: | OpenAIRE |
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