Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth

Autor:	Chavee Laomeephol, Apichai Vasuratna, Juthamas Ratanavaraporn, Sorada Kanokpanont, Jittima Amie Luckanagul, Martin Humenik, Thomas Scheibel, Siriporn Damrongsakkul
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	cell culture Polymers and Plastics fungi technology industry and agriculture Organic chemistry food and beverages General Chemistry self-assembly macromolecular substances Article QD241-441 silk fibroin spider silk hydrogel lipids (amino acids peptides and proteins)
Zdroj:	Polymers; Volume 13; Issue 23; Pages: 4182 Polymers Polymers, Vol 13, Iss 4182, p 4182 (2021)
ISSN:	2073-4360
DOI:	10.3390/polym13234182
Popis:	Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications.
Databáze:	OpenAIRE
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