Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction
| Autor: | Hugo Neves Brandão, Gabriele de Abreu Barreto, Jéssica Rebouças Silva, Danielle Devequi-Nunes, José Luiz Carneiro da Rocha, Marcelo Andrés Umsza-Guez, Danielle Figuerêdo da Silva, Bruna Aparecida Souza Machado, Valéria M. Borges |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine DPPH Chemical Composition Biochemistry 01 natural sciences Antioxidants Geographical locations Ferulic acid Mice White Blood Cells chemistry.chemical_compound Anti-Infective Agents Animal Cells Medicine and Health Sciences Food science Chromatography High Pressure Liquid Extraction Techniques Liquid Chromatography Mice Inbred BALB C Multidisciplinary Organic Compounds Chromatographic Techniques Supercritical fluid extraction Chromatography Supercritical Fluid Catechin Chemistry Physical Sciences Medicine Cellular Types Brazil Research Article Staphylococcus aureus Water activity Immune Cells Science Immunology Research and Analysis Methods Leishmania braziliensis Propolis 03 medical and health sciences Phenols Escherichia coli Animals Supercritical Fluid Extraction Flavonoids Blood Cells Ethanol 010405 organic chemistry Macrophages Organic Chemistry Extraction (chemistry) Chemical Compounds Biology and Life Sciences Cell Biology South America High Performance Liquid Chromatography 0104 chemical sciences 030104 developmental biology chemistry Alcohols People and places |
| Zdroj: | PLoS ONE, Vol 13, Iss 12, p e0207676 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Propolis is a natural product with many demonstrated biological activities and propolis extract has been used in the food, pharmaceutical and cosmetics industries. Different works have showed the variations in the chemical composition, and consequently, on the biological activity of the propolis that are associated with its type and geographic origin. Due to this study evaluated propolis extracts obtained through supercritical extraction and ethanolic extraction (conventional) in three samples of different types of propolis (red, green and brown), collected from different regions in Brazil (state of Bahia). Analyses were performed to determine the humidity, water activity, the content of total ash, proteins, lipids and fiber in raw propolis samples. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH), catechin, ferulic acid and luteolin and antimicrobial activity against two bacteria (Staphylococcus aureus and Escherichia coli) were determined for all extracts. For the green and red ethanolic extracts the anti-leishmanicidal potential was also evaluated. The physicochemical profiles showed agreement in relation to the literature. The results identified significant differences among the extracts (p>0.05), which are in conformity with their extraction method, as well as with type and botanical origin of the samples. The extraction with supercritical fluid was not efficient to obtain extracts with the highest contents of antioxidants compounds, when compared with the ethanolic extracts. The best results were shown for the extracts obtained through the conventional extraction method (ethanolic) indicating a higher selectivity for the extraction of antioxidants compounds. The red variety showed the largest biological potential, which included the content of antioxidants compounds. The results found in this study confirm the influence of the type of the raw material on the composition and characteristics of the extracts. The parameters analysis were important to characterize and evaluate the quality of the different Brazilian propolis extracts based on the increased use of propolis by the natural products industry. |
| Databáze: | OpenAIRE |
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