Role of the JAK2/STAT3 pathway in angiotensin II-induced enhanced expression of Giα proteins and hyperproliferation of aortic vascular smooth muscle cells
| Autor: | Madhu B. Anand-Srivastava, Yuan Li, Ekhtear Hossain |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
STAT3 Transcription Factor
0301 basic medicine Vascular smooth muscle Physiology GTP-Binding Protein alpha Subunits Gi-Go 030204 cardiovascular system & hematology medicine.disease_cause Muscle Smooth Vascular 03 medical and health sciences 0302 clinical medicine Rats Inbred SHR Physiology (medical) Renin–angiotensin system medicine Animals Phosphorylation Aorta Cell Proliferation Pharmacology Chemistry Angiotensin II Jak2 stat3 JAK-STAT signaling pathway Hydrogen Peroxide General Medicine Janus Kinase 2 Rats Oxidative Stress 030104 developmental biology Gene Expression Regulation cardiovascular system Cancer research hormones hormone substitutes and hormone antagonists Oxidative stress Signal Transduction |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 99:237-246 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/cjpp-2020-0415 |
| Popis: | We earlier showed that angiotensin (Ang) II-induced overexpression of Giα proteins contributes to the hyperproliferation of vascular smooth muscle cells (VSMC). In addition, the implication of the JAK2/STAT3 pathway in Ang II-induced hyperproliferation of VSMC has also been reported. However, the role of the JAK2/STAT3 pathway in Ang II-induced overexpression of Giα proteins and hyperproliferation of VSMC remains unexplored. In the present study, we show that inhibition or knockdown of the JAK2/STAT3 pathway by a specific inhibitor “cucurbitacin I” (CuI) or siRNAs attenuated Ang II-induced overexpression of Giα proteins and hyperproliferation of VSMC. In addition, the enhanced expression of cell cycle proteins induced by Ang II was also attenuated by CuI. Furthermore, Ang II-induced enhanced production of the superoxide anion (O2 –), H2O2, and NADPH oxidase activity, as well as the enhanced expression of NADPH oxidase subunits implicated in enhanced expression of Giα proteins and hyperproliferation, were also attenuated by inhibition of the JAK2/STAT3 pathway. On the other hand, Ang II-induced inhibition and augmentation of the levels of nitric oxide and peroxynitrite, respectively, in VSMC were restored to control levels by CuI. In summary, our results demonstrate that Ang II through the JAK2/STAT3 pathway increases nitroxidative stress, which contributes to the overexpression of Giα proteins and cell cycle proteins and the hyperproliferation of VSMC. |
| Databáze: | OpenAIRE |
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