Breast cancer cells and macrophages in a paracrine-juxtacrine loop
| Autor: | Devrim Pesen-Okvur, Sevgi Onal, Hamdullah Yanik, Gizem Bati-Ayaz, Merve Turker-Burhan |
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| Rok vydání: | 2021 |
| Předmět: |
animal structures
Biophysics Motility Breast Neoplasms Bioengineering 02 engineering and technology Biomaterials 03 medical and health sciences Paracrine signalling Live cell imaging Cell Movement Epidermal growth factor Cell Line Tumor Humans skin and connective tissue diseases neoplasms 030304 developmental biology 0303 health sciences Matrigel Epidermal Growth Factor integumentary system Chemistry Chemotaxis Macrophage Colony-Stimulating Factor Macrophages fungi 021001 nanoscience & nanotechnology Juxtacrine signalling Coculture Techniques Cell biology Mechanics of Materials Cancer cell Ceramics and Composites 0210 nano-technology Fetal bovine serum |
| Zdroj: | Biomaterials. 267:120412 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2020.120412 |
| Popis: | Breast cancer cells (BCC) and macrophages are known to interact via epidermal growth factor (EGF) produced by macrophages and colony stimulating factor-1 (CSF-1) produced by BCC. Despite contradictory findings, this interaction is perceived as a paracrine loop. Further, the underlying mechanism of interaction remains unclear. Here, we investigated interactions of BCC with macrophages in 2D and 3D. While both BCC and macrophages showed invasion/chemotaxis to fetal bovine serum, only macrophages showed chemotaxis to BCC in custom designed 3D cell-on-a-chip devices. These results were in agreement with gradient simulation results and ELISA results showing that macrophage-derived-EGF was not secreted into macrophage-conditioned-medium. Live cell imaging of BCC in the presence and absence of iressa showed that macrophages but not macrophage-derived matrix modulated adhesion and motility of BCC in 2D. 3D co-culture experiments in collagen and matrigel showed that BCC changed their multicellular organization in the presence of macrophages. In custom designed 3D co-culture cell-on-a-chip devices, macrophages promoted and reduced migration of BCC in collagen and matrigel, respectively. Furthermore, adherent but not suspended BCC endocytosed EGFR when in contact with macrophages. Collectively, our data revealed that macrophages showed chemotaxis towards BCC whereas BCC required direct contact to interact with macrophage-derived-EGF. Therefore, we propose that the interaction between cancer cells and macrophages is a paracrine-juxtacrine loop of CSF-1 and EGF, respectively. |
| Databáze: | OpenAIRE |
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