Lipoproteins of Haemophilus influenzae type b
| Autor: | Dwight A. Towler, Geoffrey A. Weinberg, R S Munson |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Gel electrophoresis
Molecular mass Cell growth Lipoproteins Sodium Cell chemistry.chemical_element Biology medicine.disease_cause Haemophilus influenzae Microbiology medicine.anatomical_structure chemistry Biochemistry medicine Electrophoresis Polyacrylamide Gel Tritium Bacterial outer membrane Molecular Biology Research Article Bacterial Outer Membrane Proteins |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.170.9.4161-4164.1988 |
| Popis: | Haemophilus influenzae type b Minn A produced 12 lipoproteins with apparent molecular weights of between 14,000 and 67,000. The lipoproteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of delipidated extracts of cells grown in [3H]palmitate. When the delipidated cell extracts were subjected to acid methanolysis, tritium was quantitatively recovered as palmitate and methyl palmitate, indicating that the [3H]palmitate had not been degraded and reincorporated into nonlipid material during cell growth. One of the lipoproteins comigrated with outer membrane protein (OMP) P6. OMP P6 was purified from [3H]palmitate-labeled cells. The purified protein preparation contained both amide- and ester-linked fatty acids. We conclude that (i) H. influenzae type b produces several lipoproteins, and (ii) one of these lipoproteins is OMP P6, a protein under consideration as a vaccine component. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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