Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates
Autor: | Mehmet Erinmez, Deniz Gazel, Yasemin Zer, Ayşe Büyüktaş Manay |
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Rok vydání: | 2021 |
Předmět: |
Male
Methicillin-Resistant Staphylococcus aureus 0301 basic medicine food.ingredient Turkey 030106 microbiology Vancomycin intermediate Staphylococcus aureus Brain-heart Infusion agar Microbial Sensitivity Tests medicine.disease_cause Microbiology Meropenem Hospitals University Agar plate 03 medical and health sciences 0302 clinical medicine food Vancomycin Virology Drug Resistance Bacterial Prevalence medicine Humans Agar 030212 general & internal medicine business.industry Vancomycin Resistance General Medicine Staphylococcal Infections biochemical phenomena metabolism and nutrition Anti-Bacterial Agents Culture Media Vancomycin-Resistant Staphylococcus aureus Infectious Diseases Staphylococcus aureus Therapeutic failure Female Parasitology business medicine.drug |
Zdroj: | The Journal of Infection in Developing Countries. 15:89-94 |
ISSN: | 1972-2680 |
Popis: | Introduction: Heteroresistant vancomycin intermediate Staphylococcus aureus (hVISA) testing is recommended when therapeutic failure is suspected in the clinics. In our research, we aimed to investigate the prevalence of hVISA among methicilline-resistant S. aureus (MRSA) isolates in our university hospital and compared three methods for detection of hVISA. Methodology: One hundred MRSA clinical isolates were collected in our medical microbiology laboratory between 01.04.2018 and 01.10.2019. For screening of hVISA, we used two screening agar plates and used one commercial medium; brain heart infusion agar (BHI) plates containing 4 µg/mL vancomycin and 16 g/Lt casein (BHIA-VC; Satola’s test), BHI agar plates containing 4 µg/mLvancomycin (BHIAV), and commercially obtained vancomycin resistant Enterococci (VRE) agar for detetection of hVISA. Colonies which could grow on plates were counted manually at 24th and 48th hours. Results: Among 100 MRSA isolates, 43 (43%) were found as hVISA using Satola’s test. BHIAV and VRE agar screening test results were found 70% and 4%, respectively. Finally, at the step, MIC values of 20 (47%) hVISA isolates reduced to 2 µg/mL after sub culturing for the gradient test. Conclusions: We found higher rates of hVISA comparing other studies in Turkey. Both VRE agar and BHIAV screening test failed to detect hVISA properly. Meropenem in combination with vancomycin inhibited the growth of 90% hVISA isolates in our study. |
Databáze: | OpenAIRE |
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