Environmental RNA outperforms eDNA metabarcoding in assessing impact of marine pollution: A chromium-spiked mesocosm test
| Autor: | Mattia Greco, Franck Lejzerowicz, Emanuela Reo, Antonio Caruso, Antonella Maccotta, Rodolfo Coccioni, Jan Pawlowski, Fabrizio Frontalini |
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| Přispěvatelé: | Greco M., Lejzerowicz F., Reo E., Caruso A., Maccotta A., Coccioni R., Pawlowski J., Frontalini F. |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Chromium
Environmental Engineering Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health eRNA General Medicine General Chemistry Foraminifera Mesocosm Biodiversity Settore GEO/01 - Paleontologia E Paleoecologia Ecotoxicology Pollution Settore GEO/08 - Geochimica E Vulcanologia Metabarcoding Environmental Chemistry DNA Barcoding Taxonomic RNA eDNA Ecosystem Environmental Monitoring |
| Popis: | Environmental (e)DNA metabarcoding holds great promise for biomonitoring and ecotoxicological applications. However, few studies have compared the performance of eDNA versus eRNA metabarcoding in assessing organismal response to marine pollution, in experimental conditions. Here, we performed a chromium (Cr)-spiked mesocosm experimental test on benthic foraminiferal community to investigate the effects on species diversity by analysing both eDNA and eRNA metabarcoding data across different Cr concentrations in the sediment. Foraminiferal diversity in the eRNA data showed a significant negative correlation with the Cr concentration in the sediment, while a positive response was observed in the eDNA data. The foraminiferal OTUs exhibited a higher turnover rate in eRNA than in the eDNA-derived community. Furthermore, in the eRNA samples, OTUs abundance was significantly affected by the Cr gradient in the sediment (Pseudo-R2 = 0.28, p = 0.05), while no significant trend was observed in the eDNA samples. The correlation between Cr concentration and foraminiferal diversity in eRNA datasets was stronger when the less abundant OTUs ( |
| Databáze: | OpenAIRE |
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